Li Xue, Chang Jingyang, Zhang Miaomiao, Zhou Yining, Zhang Tingting, Zhang Yiquan, Lu Renfei
Department of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People's Hospital, Nantong, China.
School of Medicine, Jiangsu University, Zhenjiang, China.
Front Microbiol. 2024 May 31;15:1340429. doi: 10.3389/fmicb.2024.1340429. eCollection 2024.
Calcium (Ca) can regulate the swarming motility and virulence of BB22. However, the effects of Ca on the physiology of RIMD2210633, whose genomic composition is quite different with that of BB22, have not been investigated. In this study, the results of phenotypic assays showed that the biofilm formation, c-di-GMP production, swimming motility, zebrafish survival rate, cytoxicity against HeLa cells, and adherence activity to HeLa cells of RIMD2210633 were significantly enhanced by Ca. However, Ca had no effect on the growth, swarming motility, capsular polysaccharide (CPS) phase variation and hemolytic activity. The RNA sequencing (RNA-seq) assay disclosed 459 significantly differentially expressed genes (DEGs) in response to Ca, including biofilm formation-associated genes and those encode virulence factors and putative regulators. DEGs involved in polar flagellum and T3SS1 were upregulated, whereas majority of those involved in regulatory functions and c-di-GMP metabolism were downregulated. The work helps us understand how Ca affects the behavior and gene expression of RIMD2210633.
钙（Ca）可调节BB22的群体运动性和毒力。然而，Ca对基因组组成与BB22差异很大的RIMD2210633生理特性的影响尚未得到研究。在本研究中，表型分析结果表明，Ca显著增强了RIMD2210633的生物膜形成、环二鸟苷酸（c-di-GMP）产生、游动运动性、斑马鱼存活率、对HeLa细胞的细胞毒性以及对HeLa细胞的黏附活性。然而，Ca对其生长、群体运动性、荚膜多糖（CPS）相变和溶血活性没有影响。RNA测序（RNA-seq）分析揭示了459个响应Ca的显著差异表达基因（DEG），包括生物膜形成相关基因以及编码毒力因子和假定调节因子的基因。参与极鞭毛和III型分泌系统1（T3SS1）的DEG上调，而参与调节功能和c-di-GMP代谢的大多数DEG下调。这项工作有助于我们了解Ca如何影响RIMD2210633的行为和基因表达。
