Zhang Miaomiao, Xue Xingfan, Li Xue, Wu Qimin, Zhang Tingting, Yang Wenhui, Hu Lingfei, Zhou Dongsheng, Lu Renfei, Zhang Yiquan
Department of Clinical Laboratory, Affiliated Nantong Hospital 3 of Nantong University, Nantong, Jiangsu, China.
School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Front Microbiol. 2023 Feb 9;14:1079653. doi: 10.3389/fmicb.2023.1079653. eCollection 2023.
Mature biofilm formation by requires exopolysaccharide (EPS), type IV pili, and capsular polysaccharide (CPS). Production of each is strictly regulated by various control pathways including quorum sensing (QS) and bis-(3'-5')-cyclic di-GMP (c-di-GMP). QsvR, an AraC-type regulator, integrates into the QS regulatory cascade direct control of the transcription of the master QS regulators, AphA and OpaR. Deletion of in wild-type or mutant backgrounds altered the biofilm formation by , suggesting that QsvR may coordinate with OpaR to control biofilm formation. Herein, we demonstrated both QsvR and OpaR repressed biofilm-associated phenotypes, c-di-GMP metabolism, and the formation of translucent (TR) colonies. QsvR restored the biofilm-associated phenotypic changes caused by mutation, and vice versa. In addition, QsvR and OpaR worked coordinately to regulate the transcription of EPS-associated genes, type IV pili genes, CPS genes and c-di-GMP metabolism-related genes. These results demonstrated how QsvR works with the QS system to regulate biofilm formation by precisely controlling the transcription of multiple biofilm formation-associated genes in .
形成成熟生物膜需要胞外多糖（EPS）、IV型菌毛和荚膜多糖（CPS）。每种物质的产生都受到包括群体感应（QS）和双（3'-5'）-环二鸟苷酸（c-di-GMP）在内的各种调控途径的严格调控。QsvR是一种AraC型调节因子，它整合到QS调控级联中，直接控制主要QS调节因子AphA和OpaR的转录。在野生型或突变背景中缺失QsvR会改变生物膜的形成，这表明QsvR可能与OpaR协同控制生物膜的形成。在此，我们证明了QsvR和OpaR都抑制了生物膜相关表型、c-di-GMP代谢以及半透明（TR）菌落的形成。QsvR恢复了由突变引起的生物膜相关表型变化，反之亦然。此外，QsvR和OpaR协同作用以调节EPS相关基因、IV型菌毛基因、CPS基因和c-di-GMP代谢相关基因的转录。这些结果证明了QsvR如何与QS系统协同工作，通过精确控制多种生物膜形成相关基因的转录来调节生物膜的形成。
