Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Van Yuzuncu Yil University, Van, Turkey.
Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Bursa Uludag University, Bursa, Turkey.
Vet Med Sci. 2024 Jul;10(4):e1509. doi: 10.1002/vms3.1509.
Anti-Müllerian Hormone (AMH) serves as a crucial parameter in assessing the reproductive herd life and ovarian reserve in cattle. Consequently, extensive research is conducted on AMH levels. Various measurement methods can be employed to determine AMH levels. However, to our knowledge, no study has been conducted on Holstein donors using the Elecsys AMH kit.
This study was designed to determine AMH levels in donors utilising the Elecsys® AMH kit and to evaluate the relationship between superovulation response parameters and AMH levels.
In this study, we measured the serum AMH levels of 36 cows using the Elecsys AMH automated assay before the superovulation protocol (1st sample) and FSH injections (2nd sample). The cows were categorised into three groups based on their AMH levels: low, medium, and high AMH.
Positive correlations were identified between AMH and parameters associated with superovulation response. The high AMH level group exhibited significantly greater numbers of corpus luteum, total embryos, transferable embryos, and grade 1 embryos compared to the medium and low AMH groups (p < 0.05) There was no significant difference between AMH levels before the superovulation protocol and FSH injections(p > 0.05). Body condition score and parity did not significantly affect AMH levels in cows (p > 0.05). Also, AMH cut-off values for the number of corpus luteum, total embryo, and transferable embryos were detected as 234, 227, and 210 pg/mL, respectively.
These findings demonstrate that a high serum AMH level has a positive influence on the superovulation response. AMH can be used as a reliable marker for the selection of donors in Holstein cows.
抗缪勒管激素（AMH）是评估牛的生殖寿命和卵巢储备的重要参数。因此，对 AMH 水平进行了广泛的研究。可以采用各种测量方法来确定 AMH 水平。但是，据我们所知，还没有关于荷斯坦供体使用 Elecsys AMH 试剂盒的研究。
本研究旨在使用 Elecsys® AMH 试剂盒确定供体的 AMH 水平，并评估超数排卵反应参数与 AMH 水平之间的关系。
在这项研究中，我们在超排卵方案（第 1 个样本）和 FSH 注射前（第 2 个样本）使用 Elecsys AMH 自动测定法测量了 36 头牛的血清 AMH 水平。根据 AMH 水平将牛分为三组：低、中、高 AMH。
AMH 与与超排卵反应相关的参数呈正相关。高 AMH 水平组的黄体数、总胚胎数、可转移胚胎数和 1 级胚胎数明显多于中、低 AMH 组（p<0.05）。超排卵方案前与 FSH 注射后的 AMH 水平无显著差异（p>0.05）。体况评分和胎次对牛的 AMH 水平无显著影响（p>0.05）。此外，黄体数、总胚胎数和可转移胚胎数的 AMH 截断值分别检测为 234、227 和 210 pg/ml。
这些发现表明，高血清 AMH 水平对超排卵反应有积极影响。AMH 可作为荷斯坦奶牛供体选择的可靠标志物。
