Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA.
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA.
Am J Vet Res. 2024 Jun 17;85(8). doi: 10.2460/ajvr.24.03.0077. Print 2024 Aug 1.
To evaluate the effects of darbepoetin on platelet population and reactivity in healthy cats (HCs) and azotemic cats with remnant kidney (RK) model-induced chronic kidney disease.
12 purpose-bred domestic shorthair cats (n = 6 HCs and n = 6 RK).
In this pilot study, all cats received darbepoetin (1 µg/kg, SC) on days 0, 7, and 14. Blood was sampled at baseline and on days 3, 10, 15, 17, 20, and 21. At each time point, a CBC was performed, platelet aggregometry was assessed by impedance and optical methods, and platelet P-selectin (CD62P) was quantified before and after thrombin stimulation. Additionally, reticulated platelets were quantified using both thiazole orange staining and proprietary analysis by the CBC analyzer. For RK cats, systemic blood pressure (BP) was serially measured.
No adverse effects of darbepoetin were seen. There was no statistically significant change in platelet count between or within groups at any time point. Hematocrit increased significantly over time in the RK but not the HC group. RBC reticulocyte numbers in both groups increased over time. Reticulated platelet percentage did not increase in either group. Differences in platelet reactivity within or between groups were not seen in the aggregometry or flow cytometric assessments. In RK cats, indirect BP did not significantly change during the study.
This preliminary investigation did not find evidence that darbepoetin administration impacted platelet number, reactivity, nor reticulated platelet count. Anemic RK cats experienced increased hematocrit and RBC reticulocytes as expected with darbepoetin therapy.
评估达贝泊汀对健康猫（HCs）和残肾（RK）模型诱导的慢性肾病的贫血猫血小板群体和反应性的影响。
12 只纯种家养短毛猫（6 只 HCs 和 6 只 RK）。
在这项初步研究中，所有猫均在第 0、7 和 14 天接受达贝泊汀（1μg/kg，SC）。在基线和第 3、10、15、17、20 和 21 天采集血液样本。在每个时间点，进行全血细胞计数（CBC），通过阻抗和光学方法评估血小板聚集，并用凝血酶刺激前后定量血小板 P-选择素（CD62P）。此外，使用噻唑橙染色和通过 CBC 分析仪进行专有分析来定量网织血小板。对于 RK 猫，连续测量全身血压（BP）。
未观察到达贝泊汀的不良反应。在任何时间点，各组之间或组内血小板计数均无统计学显著变化。在 RK 组中，血细胞比容随时间显著增加，但在 HCs 组中没有增加。两组的 RBC 网织红细胞数量随时间增加。两组的网织血小板百分比均未增加。在聚集或流式细胞术评估中，各组内或组间的血小板反应性差异不明显。在 RK 猫中，间接 BP 在研究期间没有显著变化。
这项初步研究没有发现达贝泊汀给药会影响血小板数量、反应性或网织血小板计数的证据。贫血的 RK 猫在接受达贝泊汀治疗时，如预期的那样，经历了血细胞比容和 RBC 网织红细胞增加。
