Department of Small Animal Medicine and Surgery, University of Georgia College of Veterinary Medicine, 2200 College Station Road, Athens, Georgia 30602, USA.
Department of Pathology, University of Georgia College of Veterinary Medicine, 2200 College Station Road, Athens, Georgia 30602, USA.
J Vet Intern Med. 2021 Jul;35(4):1754-1762. doi: 10.1111/jvim.16169. Epub 2021 May 16.
Vincristine might increase circulating platelet numbers but the functional capacity of these newly released platelets is unknown.
To evaluate and compare the functionality of mature and immature (reticulated) platelets after a single intravenous dose of vincristine in dogs.
Ten healthy purpose-bred dogs.
Dogs prospectively received a single IV injection of 0.02 mg/kg vincristine or 0.9% saline. Before and after treatment on days 3, 5, and 7, platelets (resting and after thrombin stimulation) were assessed by flow cytometric determination of P-selectin (CD62P) expression. Reticulated platelets were distinguished using thiazole orange (TO) staining.
Relative to saline, vincristine administration increased platelet count from day 0 to day 7 (225 ± 58 to 273 ± 65 × 10 /μL, vs 299 ± 76.4 to 214 ± 20 × 10 /μL, P = .01) and increased percentage of reticulated platelets from day 0 to day 5 (3.9 ± 1.5% to 6.1 ± 1.6%, P = .02). On all days, reticulated platelets had greater resting expression of CD62P than did mature platelets (49.6 ± 4% vs 10.2 ± 1%, P ≤ .001). Across all days, CD62P expression by reticulated platelets in the vincristine and saline-treated groups was not different when unstimulated (P = .7) or after thrombin stimulation (P = .33).
Reticulated platelets released in response to vincristine administration function similarly to mature platelets.
长春新碱可能会增加循环血小板数量，但这些新释放的血小板的功能能力尚不清楚。
评估并比较犬单次静脉注射长春新碱后成熟和未成熟（网织）血小板的功能。
10 只健康的专门繁殖的狗。
狗前瞻性地接受 0.02mg/kg长春新碱或 0.9%生理盐水单次 IV 注射。在治疗前和治疗后第 3、5 和 7 天，通过流式细胞术测定 P-选择素（CD62P）表达评估血小板（静息和凝血酶刺激后）。使用噻唑橙（TO）染色区分网织血小板。
与生理盐水相比，长春新碱给药从第 0 天到第 7 天增加了血小板计数（225±58 至 273±65×10 /μL，与 299±76.4 至 214±20×10 /μL，P=0.01），并从第 0 天增加了网织血小板的百分比至第 5 天（3.9±1.5%至 6.1±1.6%，P=0.02）。在所有日子里，与成熟血小板相比，网织血小板的 CD62P 静息表达更高（49.6±4% 与 10.2±1%，P≤0.001）。在所有日子里，未刺激时（P=0.7）或刺激后（P=0.33），长春新碱和生理盐水处理组的网织血小板的 CD62P 表达无差异。
长春新碱给药后释放的网织血小板的功能与成熟血小板相似。
