Yang Lianzhi, Yu Pan, Wang Juanjuan, Zhao Taixia, Zhao Yong, Pan Yingjie, Chen Lanming
Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of China, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Foods. 2024 May 27;13(11):1674. doi: 10.3390/foods13111674.
can cause acute gastroenteritis, wound infections, and septicemia in humans. The overuse of antibiotics in aquaculture may lead to a high incidence of the multidrug-resistant (MDR) pathogen. Nevertheless, the genome evolution of in aquatic animals and the mechanism of its antibiotic tolerance remain to be further deciphered. Here, we investigated the molecular basis of the antibiotic tolerance of isolates ( = 3) originated from shellfish and crustaceans using comparative genomic and transcriptomic analyses. The genome sequences of the isolates were determined (5.0-5.3 Mb), and they contained 4709-5610 predicted protein-encoding genes, of which 823-1099 genes were of unknown functions. Comparative genomic analyses revealed a number of mobile genetic elements (MGEs, = 69), antibiotic resistance-related genes ( = 7-9), and heavy metal tolerance-related genes ( = 2-4). The isolates were resistant to sub-lethal concentrations (sub-LCs) of ampicillin (AMP, 512 μg/mL), kanamycin (KAN, 64 μg/mL), and streptomycin (STR, 16 μg/mL) ( < 0.05). Comparative transcriptomic analyses revealed that there were significantly altered metabolic pathways elicited by the sub-LCs of the antibiotics ( < 0.05), suggesting the existence of multiple strategies for antibiotic tolerance in . The results of this study enriched the genome database and should be useful for controlling the MDR pathogen worldwide.
可导致人类急性肠胃炎、伤口感染和败血症。水产养殖中抗生素的过度使用可能导致多重耐药（MDR）病原体的高发病率。然而，水生动物中该病原体的基因组进化及其抗生素耐受性机制仍有待进一步破译。在此，我们使用比较基因组学和转录组学分析，研究了来自贝类和甲壳类动物的3株该病原体分离株抗生素耐受性的分子基础。测定了这3株分离株的基因组序列（5.0 - 5.3 Mb），它们包含4709 - 5610个预测的蛋白质编码基因，其中823 - 1099个基因功能未知。比较基因组分析揭示了一些移动遗传元件（MGEs，共69个）、抗生素抗性相关基因（共7 - 9个）和重金属耐受性相关基因（共2 - 4个）。这些分离株对氨苄青霉素（AMP，512 μg/mL）、卡那霉素（KAN，64 μg/mL）和链霉素（STR，16 μg/mL）的亚致死浓度（sub - LCs）具有抗性（P < 0.05）。比较转录组分析表明，抗生素亚致死浓度引发了显著改变的代谢途径（P < 0.05），这表明该病原体存在多种抗生素耐受策略。本研究结果丰富了该病原体的基因组数据库，对全球控制多重耐药病原体应具有重要意义。
