College of Chemistry, Green Catalysis Center, Zhengzhou University, Zhengzhou 450001, PR China.
Department of Orthopedics The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, PR China.
Food Chem. 2024 May 30;441:138333. doi: 10.1016/j.foodchem.2023.138333. Epub 2024 Jan 3.
A sensitive signal-on photoelectrochemical aptasensor for antibiotic determination was constructed based on the energy level matching between ferrocene and CuInS. P-type CuInS microflower was complexed with reduced graphene oxide (CuInS/rGO) to get photocathode current with good photoelectric conversion efficiency and stability. Then, hairpin DNA (HP) was covalently bonded to the electrode surface. A triple helix DNA (THMS) was used as a molecular switch. After the specific recognition between target and THMS in homogeneous solution, ferrocene labeled probe (Fc-T2) was released. Finally, Fc-T2 was captured by the HP, which leaded the obvious increase of photocurrent for the energy level matching between ferrocene and CuInS. The increase of the photocurrent signal was proportional to the concentration of target amoxicillin (AMOX), the linear range was 100 fM-100 nM with detection limit of 19.57 fM. Meanwhile, the method has been successfully applied for milk and lake water samples analysis with satisfactory results.
基于二茂铁与 CuInS 之间的能级匹配，构建了一种用于抗生素测定的灵敏信号光电化学适体传感器。将 P 型 CuInS 微花与还原氧化石墨烯（CuInS/rGO）复合，以获得具有良好光电转换效率和稳定性的光电流。然后，发夹 DNA（HP）被共价键合到电极表面。三螺旋 DNA（THMS）被用作分子开关。在均相溶液中目标物与 THMS 之间进行特异性识别后，释放出标记有二茂铁的探针（Fc-T2）。最后，Fc-T2 被 HP 捕获，导致二茂铁与 CuInS 之间的能级匹配产生明显的光电流增加。光电流信号的增加与阿莫西林（AMOX）的浓度成正比，线性范围为 100 fM-100 nM，检测限为 19.57 fM。同时，该方法已成功应用于牛奶和湖水样品的分析，结果令人满意。
