Chen Lili, Nie Menghan, Yang Jing, Zhang Weibin, Hsiang Tom, Jiang Yuji, Xie Baogui, Chen Bingzhi
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Antioxidants (Basel). 2025 May 13;14(5):586. doi: 10.3390/antiox14050586.
This study investigated the structure-activity relationships between SePEPs (selenium-enriched peptides) and PEPs (selenium-free peptides) and compared the antioxidant activities of SePEPs and PEPs. The results showed that SePEPs exhibited higher antioxidant activity than PEPs at the same molecular weight, with the molecular weights of 0-3500 Da exhibiting the highest in vitro antioxidant activity. Chelation between selenium and peptides led to a more compact structure and increased particle density in SePEPs. A spectroscopic analysis revealed new peaks and redshifts in SePEPs, along with a higher content of hydrophobic amino acids than PEPs. A molecular interaction modeling analysis indicated that hydrogen bonding and hydrophobic interactions primarily drove the binding between selenium-containing peptides and 1,1-diphenyl-2-picrylhydrazyl (DPPH). Moreover, the solid-phase synthesized MSePGP exhibited significantly greater antioxidant activity than glutathione at high concentrations. At 10 mg/mL, the DPPH radical scavenging rate of MSePGP was 68.5 ± 2.2%. These findings provide a theoretical basis for the design and synthesis of selenium-enriched peptides with enhanced antioxidant properties.
本研究调查了富硒肽（SePEPs）和无硒肽（PEPs）之间的构效关系，并比较了SePEPs和PEPs的抗氧化活性。结果表明，在相同分子量下，SePEPs比PEPs表现出更高的抗氧化活性，其中分子量为0 - 3500 Da的在体外抗氧化活性最高。硒与肽之间的螯合导致SePEPs结构更紧凑且颗粒密度增加。光谱分析显示SePEPs出现新峰和红移，并且其疏水氨基酸含量高于PEPs。分子相互作用建模分析表明，氢键和疏水相互作用主要驱动含硒肽与1,1 - 二苯基 - 2 - 苦基肼（DPPH）之间的结合。此外，固相合成的MSePGP在高浓度下表现出比谷胱甘肽显著更高的抗氧化活性。在10 mg/mL时，MSePGP的DPPH自由基清除率为68.5±2.2%。这些发现为设计和合成具有增强抗氧化性能的富硒肽提供了理论依据。
