Department of Restorative Dental Sciences, College of Dentistry, King Saud University, P.O. Box 60169, Riyadh 11612, Saudi Arabia.
Therapeutics & Biomarker Discovery for Clinical Applications, Cell Therapy & Immunobiology Department, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
Cells. 2024 May 28;13(11):932. doi: 10.3390/cells13110932.
Periodontal disease is characterized by inflammation and bone loss. Central to its pathogenesis is the dysregulated inflammatory response, complicating regenerative therapies. Mesenchymal stem cells (MSCs) hold significant promise in tissue repair and regeneration. This study investigated the effects of specialized pro-resolving mediators (SPMs), Resolvin E1 (RvE1) and Maresin 1 (MaR1), on the osteogenic differentiation of human bone marrow-derived MSCs under inflammatory conditions. The stem cells were treated with SPMs in the presence of lipopolysaccharide (LPS) to simulate an inflammatory environment. Osteogenic differentiation was assessed through alkaline phosphatase activity and alizarin red staining. Proteomic analysis was conducted to characterize the protein expression profile changes, focusing on proteins related to osteogenesis and osteoclastogenesis. Treatment with RvE1 and MaR1, both individually and in combination, significantly enhanced calcified deposit formation. Proteomic analysis revealed the differential expression of proteins associated with osteogenesis and osteoclastogenesis, highlighting the modulatory impact of SPMs on bone metabolism. RvE1 and MaR1 promote osteogenic differentiation of hBMMSCs in an inflammatory environment, with their combined application yielding synergistic effects. This study provides insights into the therapeutic potential of SPMs in enhancing bone regeneration, suggesting a promising avenue for developing regenerative therapies for periodontal disease and other conditions characterized by inflammation-induced bone loss.
牙周病的特征为炎症和骨丧失。其发病机制的核心是失调的炎症反应，这使再生疗法变得复杂。间充质干细胞（MSCs）在组织修复和再生方面具有重要的应用前景。本研究探讨了特殊的促解决介质（SPMs），即 Resolvin E1（RvE1）和 Maresin 1（MaR1），在炎症条件下对人骨髓来源的间充质干细胞（hBMMSCs）成骨分化的影响。在存在脂多糖（LPS）的情况下，用 SPMs 处理干细胞，以模拟炎症环境。通过碱性磷酸酶活性和茜素红染色评估成骨分化。进行蛋白质组学分析以描述蛋白质表达谱的变化，重点关注与成骨和破骨细胞生成相关的蛋白质。RvE1 和 MaR1 的单独和联合处理均显著增强了钙化沉积物的形成。蛋白质组学分析揭示了与成骨和破骨细胞生成相关的蛋白质的差异表达，突出了 SPMs 对骨代谢的调节作用。RvE1 和 MaR1 促进 hBMMSCs 在炎症环境中的成骨分化，其联合应用具有协同作用。本研究为 SPMs 在增强骨再生方面的治疗潜力提供了新的见解，为开发治疗牙周病和其他以炎症诱导骨丧失为特征的疾病的再生疗法提供了新的途径。
