Singh G, Katyal S L, Ward J M, Gottron S A, Wong-Chong M L, Riley E J
J Histochem Cytochem. 1985 Jun;33(6):564-8. doi: 10.1177/33.6.3889141.
The reactivity of rabbit antisera to rat lung secretory proteins with other rodent species was evaluated by immunocytochemistry. Rabbit anti-rat surfactant apoprotein antiserum reacts with the cytoplasm of rat, mouse, and hamster type II pneumocytes and is specific for these cells. Rabbit antiserum to rat Clara cell secretory proteins stains rat, mouse, and hamster Clara cells. Rabbit antisera specific to the two antigenic types of rat Clara cell antigens were also both reactive with rat, mouse, and hamster Clara cells. An antiserum to the non-serum proteins of hamster lung lavage was also prepared and shown to be specifically reactive with hamster Clara cells. The availability of specific reagents for secretory proteins of rodent lungs is expected to facilitate studies of the respective cell types in various pathologic states.
通过免疫细胞化学方法评估了兔抗大鼠肺分泌蛋白抗血清与其他啮齿类动物的反应性。兔抗大鼠表面活性物质载脂蛋白抗血清与大鼠、小鼠和仓鼠的II型肺细胞的细胞质发生反应，且对这些细胞具有特异性。兔抗大鼠克拉拉细胞分泌蛋白抗血清可使大鼠、小鼠和仓鼠的克拉拉细胞染色。针对大鼠克拉拉细胞两种抗原类型的兔抗血清也都与大鼠、小鼠和仓鼠的克拉拉细胞发生反应。还制备了一种针对仓鼠肺灌洗非血清蛋白的抗血清，结果显示其与仓鼠克拉拉细胞具有特异性反应。啮齿类动物肺分泌蛋白特异性试剂的可得性有望促进对各种病理状态下相应细胞类型的研究。
