Liposits Z, Görcs T, Trombitás K
J Histochem Cytochem. 1985 Jun;33(6):604-10. doi: 10.1177/33.6.3889144.
Serotonin immunoreactive structures of the rat central nervous system (CNS) were detected by the recently developed silver-intensified peroxidase-antiperoxidase complex (SI-PAP) method at both the light and electron microscopic levels. The silver postintensification of the diaminobenzidine (DAB) chromogen increased the sensitivity of the original PAP method, resulting in a very Golgi-like appearance of serotonin-immunopositive neuronal elements. The metallic silver and gold deposited onto DAB-labeled organelles, filling out the whole immunoreactive neuron, assures the easy tracing of thin neuronal processes far from the cell body. At the ultrastructural level, metallic grains were seen over immunolabeled structures only, proving the specificity of the silver method. In neurons of the dorsal raphe nucleus, free ribosomes, endoplasmic reticulum, and granules (80-100 nm in diameter) were labeled. Immunoreactive, e.g., serotoninergic, dendrites were seen to receive afferent terminals. The increased electron density of the intensified immunolabel facilitates the ultrastructural recognition of even weakly labeled profiles, while its metallic components (Ag and Au) provide a base for X-ray analysis of the immunolabeled biological specimen.
采用最近开发的银增强过氧化物酶-抗过氧化物酶复合物（SI-PAP）方法，在光学和电子显微镜水平上检测大鼠中枢神经系统（CNS）中的5-羟色胺免疫反应性结构。二氨基联苯胺（DAB）色原的银后增强提高了原始PAP方法的灵敏度，使5-羟色胺免疫阳性神经元成分呈现出非常类似高尔基染色的外观。沉积在DAB标记细胞器上的金属银和金填充了整个免疫反应性神经元，确保了远离细胞体的细神经突起易于追踪。在超微结构水平上，仅在免疫标记结构上可见金属颗粒，证明了银法的特异性。在中缝背核的神经元中，游离核糖体、内质网和颗粒（直径80-100nm）被标记。可见免疫反应性的，如5-羟色胺能的树突接受传入终末。增强免疫标记的电子密度增加有助于对即使是弱标记的轮廓进行超微结构识别，而其金属成分（Ag和Au）为免疫标记生物标本的X射线分析提供了基础。
