Li Shu, Yang Qiao, Li Maohua, Lan Yue, Song Zhaobin
Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Animals (Basel). 2024 May 27;14(11):1586. doi: 10.3390/ani14111586.
The hybrid yellow catfish exhibits advantages over pure yellow catfish in terms of fast growth, fast development, a high feeding rate, and strong immunity; additionally, it is almost sterile, thus ensuring the conservation of the genetic stock of fish populations. To investigate the sterility mechanism in hybrid yellow catfish (♀) × ♂)), the mRNA and miRNA of the gonads of , , and a hybrid yellow catfish were analyzed to characterize the differentially expressed genes; this was carried out to help establish gene expression datasets to assist in the further determination of the mechanisms of genetic sterility in hybrid yellow catfish. In total, 1709 DEGs were identified between the hybrid and two pure yellow catfishes. A KEGG pathway analysis indicated that several genes related to reproductive functions were upregulated, including those involved in the cell cycle, progesterone-mediated oocyte maturation, and oocyte meiosis, and genes associated with ECM-receptor interaction were downregulated. The spermatogenesis-related GO genes , , and were identified as being downregulated DEGs in the hybrid yellow catfish. Sixty-three DEmiRNAs were identified between the hybrid and the two pure yellow catfish species. The upregulated DEmiRNAs and were found to target the spermatogenesis-related genes and , respectively, playing a negative regulatory role, which may underscore the miRNA-mRNA regulatory mechanism of sterility in hybrid yellow catfish. The differential expression of , , and and their target genes , , and , implicated in reproductive processes, was verified via qRT-PCR, consistent with the transcriptome sequencing expression trends. This study provides deep insights into the mechanism of hybrid sterility in vertebrate groups, thereby contributing to achieving a better understanding and management of fish conservation related to hybrid sterility.
杂交黄颡鱼在生长速度快、发育快、摄食率高和免疫力强等方面比纯黄颡鱼具有优势；此外，它几乎不育，从而确保了鱼类种群遗传资源的保存。为了研究杂交黄颡鱼（♀）×♂）的不育机制，分析了、和杂交黄颡鱼性腺的mRNA和miRNA，以表征差异表达基因；这样做是为了帮助建立基因表达数据集，以协助进一步确定杂交黄颡鱼遗传不育的机制。总共在杂交黄颡鱼和两种纯黄颡鱼之间鉴定出1709个差异表达基因。KEGG通路分析表明，几个与生殖功能相关的基因上调，包括参与细胞周期、孕酮介导的卵母细胞成熟和卵母细胞减数分裂的基因，而与细胞外基质-受体相互作用相关的基因下调。在杂交黄颡鱼中，与精子发生相关的GO基因、和被鉴定为下调的差异表达基因。在杂交黄颡鱼和两种纯黄颡鱼之间鉴定出63个差异表达的miRNA。上调的差异表达miRNA和分别被发现靶向与精子发生相关的基因和，发挥负调控作用，这可能突出了杂交黄颡鱼不育的miRNA-mRNA调控机制。通过qRT-PCR验证了参与生殖过程的、和及其靶基因、和的差异表达，与转录组测序表达趋势一致。本研究为脊椎动物群体中杂交不育的机制提供了深入见解，从而有助于更好地理解和管理与杂交不育相关的鱼类保护。
