Dong Xuan, Xie Jingmei, Wang Liying, Li Xuan, Lou Haoyu, Wang Guohao, Huang Jie
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao 266071, China.
Jiangsu Shufeng Aquatic Seed Industry Co., Ltd., Gaoyou 255654, China.
Animals (Basel). 2024 May 29;14(11):1600. doi: 10.3390/ani14111600.
Acute hepatopancreatic necrosis disease (AHPND) poses a significant threat to shrimp aquaculture worldwide, necessitating the accurate and rapid detection of the pathogens. However, the increasing number of species that cause the disease makes diagnosis and control more difficult. This study focuses on developing a monoclonal antibody against the insect-related (Pir) toxin B (PirB), a pivotal virulence factor in AHPND-causing , and establishing a colloidal gold immunochromatographic assay for the enhanced early diagnosis and monitoring of AHPND. Monoclonal antibodies targeting PirB were developed and utilized in the preparation of colloidal-gold-labeled antibodies for the immunochromatographic assay. The specificity and sensitivity of the assay were evaluated through various tests, including antibody subclass detection, affinity detection, and optimal labeling efficiency assessment. The developed PirB immunochromatographic test strips exhibited a good specificity, as demonstrated by the positive detection of AHPND-causing and negative results for non-AHPND-causing . The study highlights the potential of the developed monoclonal antibody and immunochromatographic assay for the effective detection of AHPND-causing . Further optimization is needed to enhance the sensitivity of the test strips for improved practical applications in disease prevention and control in shrimp aquaculture.
急性肝胰腺坏死病（AHPND）对全球对虾养殖业构成重大威胁，因此需要准确快速地检测病原体。然而，引发该病的物种数量不断增加，使得诊断和防控变得更加困难。本研究聚焦于开发一种针对与昆虫相关的（Pir）毒素B（PirB）的单克隆抗体，PirB是导致AHPND的关键毒力因子，并建立一种胶体金免疫层析法以加强对AHPND的早期诊断和监测。针对PirB的单克隆抗体被开发出来，并用于制备用于免疫层析测定的胶体金标记抗体。通过各种测试评估该测定的特异性和灵敏度，包括抗体亚类检测、亲和力检测和最佳标记效率评估。所开发的PirB免疫层析试纸条表现出良好的特异性，对导致AHPND的菌株检测呈阳性，对非导致AHPND的菌株检测呈阴性。该研究突出了所开发的单克隆抗体和免疫层析测定法在有效检测导致AHPND的菌株方面的潜力。需要进一步优化以提高试纸条的灵敏度，以便在对虾养殖疾病预防和控制中获得更好的实际应用效果。
