Li Rui, Wang Lizhi, Chen Binlong, Zhang Yi, Qi Pei
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Local Characteristic Goat, Xi Chang University, Xichang 615012, China.
Animals (Basel). 2024 May 29;14(11):1616. doi: 10.3390/ani14111616.
The objective of this experiment was to delve into the impacts of transportation on goats. Sixteen healthy goats were selected as experimental animals; these goats were transported at a speed ranging from 35 to 45 km/h for 20 h. The changes in the physiological indexes, blood physiological indexes, biochemical indexes, rumen fermentation indexes, and rumen microbial structure composition of goats before and after transportation were measured. The results showed that after transportation, the contents of IgM, IgA, IgG, and Thyroxine decreased very significantly, while the contents of propionic acid, Hemoglobin and Epinephrine significantly increased, and the contents of VFA, acetic acid, butyric acid, isobutyric acid, isovaleric acid, LPS, IL-1β, IL-6, TNF-α, Major Acute Phase Protein, protein carbonyl, and cortisol increased very significantly. There was no significant difference in α-diversity and β-diversity, and the relative abundance of rumen microorganisms was not significantly different at either phylum or genus levels. The experimental findings revealed that continuous transportation for a duration of 20 h can induce a severe stress response in goats, leading to compromised immune function, diminished antioxidant capacity, escalated inflammatory response, and altered rumen fermentation indices. However, the experiment did not reveal any significant impact on the structure and composition of the rumen microbiota.
本实验的目的是探究运输对山羊的影响。选择16只健康山羊作为实验动物；这些山羊以35至45公里/小时的速度运输20小时。测定运输前后山羊的生理指标、血液生理指标、生化指标、瘤胃发酵指标和瘤胃微生物结构组成的变化。结果表明，运输后，IgM、IgA、IgG和甲状腺素含量极显著降低，而丙酸、血红蛋白和肾上腺素含量显著升高，挥发性脂肪酸、乙酸、丁酸、异丁酸、异戊酸、脂多糖、白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α、主要急性期蛋白、蛋白质羰基和皮质醇含量极显著升高。α多样性和β多样性无显著差异，瘤胃微生物的相对丰度在门水平和属水平上均无显著差异。实验结果表明，持续运输20小时可诱导山羊产生严重的应激反应，导致免疫功能受损、抗氧化能力下降、炎症反应加剧和瘤胃发酵指标改变。然而，实验未发现对瘤胃微生物群的结构和组成有任何显著影响。
