Law B J, Marks M I
J Infect Dis. 1985 Jun;151(6):988-94. doi: 10.1093/infdis/151.6.988.
Administration of antibody to Escherichia coli strain J5 reduces mortality in patients with gram-negative septic shock. Limited evidence suggests that this antibody is present in the serum of healthy individuals and deficient in persons with malignancy; however, the age-related prevalence of serum antibody to strain J5 in normal subjects is unknown. We developed an ELISA method to measure IgG and IgM antibody to purified J5 lipopolysaccharide (LPS) in sera from 33 maternal-newborn cord pairs, 40 neonates, and 253 individuals aged from one day to twenty years. Reciprocal geometric mean titers for serum antibody to strain J5 LPS were as follows: maternal-IgG, 12.3; IgM, 174; one month old or less-IgG, 8.3; IgM, 5.6; one to 24 months old-IgG, 10.5; IgM, 44.7; greater than 24 months old -IgG, 16.2; IgM, 200. We conclude that human serum antibody to J5 LPS is predominantly IgM and that it is usually present by two years of age. The ELISA method should provide a valuable tool in studying the relation of core glycolipid antibody to immunity and therapy of gram-negative bacterial infections.
给予抗大肠杆菌J5菌株抗体可降低革兰氏阴性败血症休克患者的死亡率。有限的证据表明，这种抗体存在于健康个体的血清中，而在恶性肿瘤患者中缺乏；然而，正常受试者中血清抗J5菌株抗体的年龄相关患病率尚不清楚。我们开发了一种ELISA方法，用于测量来自33对母婴脐带血、40名新生儿以及253名年龄从1天至20岁个体的血清中针对纯化J5脂多糖（LPS）的IgG和IgM抗体。针对J5 LPS菌株血清抗体的倒数几何平均滴度如下：母体IgG为12.3；IgM为174；1个月及以下婴儿IgG为8.3；IgM为5.6；1至24个月大婴儿IgG为10.5；IgM为44.7；大于24个月大个体IgG为16.2；IgM为200。我们得出结论，人血清中针对J5 LPS的抗体主要是IgM，且通常在两岁时出现。ELISA方法应为研究核心糖脂抗体与革兰氏阴性细菌感染免疫及治疗之间的关系提供一个有价值的工具。
