College of Plant Protection, Department of Plant Pathology, Shenyang Agricultural University, Shenyang 110866, China.
Liaoning Academy of Agricultural Sciences, Shenyang 110101, China.
Int J Mol Sci. 2024 May 26;25(11):5787. doi: 10.3390/ijms25115787.
Transcription factors are key molecules involved in transcriptional and post-transcriptional regulation in plants and play an important regulatory role in resisting biological stress. In this study, we identified a regulatory factor, , mediating rice response to () AG1-IA infection. The expression of affects rice infection. knockout and overexpressed rice plants were constructed, and the phenotypes of mutant and wild-type (WT) plants showed that negatively regulated rice resistance to rice sheath blight. However, it was speculated that OsZF8 plays a regulatory role at the protein level. The interacting protein PRB1 of OsZF8 was screened using the yeast two-hybrid and bimolecular fluorescence complementation test. The results showed that OsZF8 effectively inhibited PRB1-induced cell death in tobacco cells, and molecular docking results showed that PRB1 had a strong binding effect with OsZF8. Further, the binding ability of OsZF8-PRB1 to ergosterol was significantly reduced when compared with the PRB1 protein. These findings provide new insights into elucidating the mechanism of rice resistance to rice sheath blight.
转录因子是参与植物转录和转录后调控的关键分子，在抵抗生物胁迫中发挥重要的调节作用。在本研究中，我们鉴定了一个调控因子 ，介导水稻对 （）AG1-IA 感染的反应。 的表达影响 水稻的感染。构建了 的敲除和过表达水稻植株，突变体和野生型（WT）植株的表型表明 负调控水稻对稻瘟病的抗性。然而，据推测 OsZF8 在蛋白质水平上发挥调节作用。使用酵母双杂交和双分子荧光互补试验筛选了 OsZF8 的互作蛋白 PRB1。结果表明，OsZF8 能有效抑制烟草细胞中 PRB1 诱导的细胞死亡，分子对接结果表明 PRB1 与 OsZF8 具有很强的结合作用。进一步的，与 PRB1 蛋白相比，OsZF8-PRB1 与麦角固醇的结合能力显著降低。这些发现为阐明水稻抗稻瘟病的机制提供了新的见解。
