Department of Experimental Medicine, University of Salento, 73100 Lecce, Italy.
Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, 73100 Lecce, Italy.
Int J Mol Sci. 2024 May 30;25(11):6000. doi: 10.3390/ijms25116000.
N-methylpyridinium (NMP) is produced through the pyrolysis of trigonelline during the coffee bean roasting process. Preliminary studies suggest that NMP may have health benefits, thanks to its antioxidant properties. Based on this background, the aim of this study was to evaluate whether NMP could have a protective effect against LPS-induced neuroinflammation in human glioblastoma cells (U87MG). With this aim, U87MG cells were pre-treated with NMP (0.5 μM) for 1 h and then exposed to LPS (1 μg/mL) for 24 h. Our findings show that NMP attenuates LPS-induced neuroinflammation by reducing the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α and IL-6, through the inhibition of the NF-κB signaling pathway, which is critical in regulating inflammatory responses. NMP is able to suppress the activation of the NF-κB signaling pathway, suggesting its potential in preventing neuroinflammatory conditions. These outcomes support the notion that regular consumption of NMP, possibly through coffee consumption, may offer protection against neuroinflammatory states implicated in neurological disorders.
N-甲基吡啶鎓（NMP）是咖啡豆烘焙过程中天冬氨酸分解生成的。初步研究表明，由于其抗氧化特性，NMP 可能对健康有益。基于这一背景，本研究旨在评估 NMP 是否可以对人神经胶质瘤细胞（U87MG）中 LPS 诱导的神经炎症产生保护作用。为此，用 NMP（0.5μM）预处理 U87MG 细胞 1 小时，然后用 LPS（1μg/mL）处理 24 小时。我们的研究结果表明，NMP 通过抑制 NF-κB 信号通路来减轻 LPS 诱导的神经炎症，从而减少促炎细胞因子（如 IL-1β、TNF-α 和 IL-6）的表达，该通路在调节炎症反应中起关键作用。NMP 能够抑制 NF-κB 信号通路的激活，这表明它在预防神经炎症方面具有潜力。这些结果支持了这样一种观点，即定期摄入 NMP（可能通过喝咖啡）可能有助于预防与神经退行性疾病相关的神经炎症状态。
