Laboratoire de BioImagerie et Pathologies, UMR CNRS 7021, ITI InnoVec, Université de Strasbourg, 67401 Illkirch, France.
CNRS, UMR 7242, ITI InnoVec, ESBS, 67412 Illkirch, France.
Int J Mol Sci. 2024 May 30;25(11):6013. doi: 10.3390/ijms25116013.
The pyoverdine siderophore is produced by to access iron. Its synthesis involves the complex coordination of four nonribosomal peptide synthetases (NRPSs), which are responsible for assembling the pyoverdine peptide backbone. The precise cellular organization of these NRPSs and their mechanisms of interaction remain unclear. Here, we used a combination of several single-molecule microscopy techniques to elucidate the spatial arrangement of NRPSs within pyoverdine-producing cells. Our findings reveal that PvdL differs from the three other NRPSs in terms of localization and mobility patterns. PvdL is predominantly located in the inner membrane, while the others also explore the cytoplasmic compartment. Leveraging the power of multicolor single-molecule localization, we further reveal co-localization between PvdL and the other NRPSs, suggesting a pivotal role for PvdL in orchestrating the intricate biosynthetic pathway. Our observations strongly indicates that PvdL serves as a central orchestrator in the assembly of NRPSs involved in pyoverdine biosynthesis, assuming a critical regulatory function.
绿脓菌素铁载体由 产生以获取铁。其合成涉及四个非核糖体肽合成酶（NRPS）的复杂协调，这些酶负责组装绿脓菌素肽骨架。这些 NRPS 的精确细胞组织及其相互作用机制仍不清楚。在这里，我们使用了几种单分子显微镜技术的组合来阐明产绿脓菌素细胞内 NRPS 的空间排列。我们的研究结果表明，PvdL 在定位和迁移模式方面与其他三个 NRPS 不同。PvdL 主要位于内膜，而其他则也探索细胞质区室。利用多色单分子定位的强大功能，我们进一步揭示了 PvdL 与其他 NRPS 之间的共定位，表明 PvdL 在协调复杂的生物合成途径中起着关键作用。我们的观察结果强烈表明，PvdL 作为参与绿脓菌素生物合成的 NRPS 组装的中央协调者，发挥着关键的调节功能。
