Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh.
Graduate School of Nanobiosciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Kanagawa, Japan.
Molecules. 2024 May 27;29(11):2531. doi: 10.3390/molecules29112531.
A Cucurbita phloem exudate lectin (CPL) from summer squash () fruits was isolated and its sugar-binding properties and biological activities were studied. The lectin was purified by affinity chromatography and the hemagglutination assay method was used to determine its pH, heat stability, metal-dependency and sugar specificity. Antimicrobial and anticancer activities were also studied by disc diffusion assays and in vivo and in vitro methods. The molecular weight of CPL was 30 ± 1 KDa and it was stable at different pH (5.0 to 9.0) and temperatures (30 to 60 °C). CPL recovered its hemagglutination activity in the presence of Ca. 4-nitrophenyl-α-D-glucopyranoside, lactose, rhamnose and -acetyl-D-glucosamine strongly inhibited the activity. With an LC value of 265 µg/mL, CPL was moderately toxic and exhibited bacteriostatic, bactericidal and antibiofilm activities against different pathogenic bacteria. It also exhibited marked antifungal activity against and agglutinated spores. In vivo antiproliferative activity against Ehrlich ascites carcinoma (EAC) cells in Swiss albino mice was observed when CPL exerted 36.44% and 66.66% growth inhibition at doses of 3.0 mg/kg/day and 6.0 mg/kg/day, respectively. A 12-day treatment by CPL could reverse their RBC and WBC counts as well as restore the hemoglobin percentage to normal levels. The MTT assay of CPL performed against human breast (MCF-7) and lung (A-549) cancer cell lines showed 29.53% and 18.30% of inhibitory activity at concentrations of 128 and 256 µg/mL, respectively.
从夏南瓜（）果实中分离出一种葫芦素属植物凝集素（CPL），并研究了其糖结合特性和生物活性。该凝集素通过亲和层析进行纯化，并用血凝试验法测定其 pH 值、热稳定性、金属依赖性和糖特异性。还通过圆盘扩散试验以及体内和体外方法研究了其抗菌和抗癌活性。CPL 的分子量为 30 ± 1 kDa，在不同 pH 值（5.0 至 9.0）和温度（30 至 60°C）下均稳定。CPL 在存在 Ca 的情况下恢复其血凝活性。4-硝基苯基-α-D-吡喃葡萄糖苷、乳糖、鼠李糖和-N-乙酰-D-葡萄糖胺强烈抑制其活性。CPL 的 LC 值为 265 µg/mL，具有中等毒性，对不同致病菌表现出抑菌、杀菌和抗生物膜活性。它还对 和 孢子表现出明显的抗真菌活性。当 CPL 在 3.0 mg/kg/天和 6.0 mg/kg/天的剂量下分别表现出 36.44%和 66.66%的生长抑制作用时，在瑞士白化病小鼠体内对艾氏腹水癌（EAC）细胞表现出明显的抗增殖活性。CPL 对红细胞和白细胞计数的体内抗增殖活性在 12 天的治疗后可恢复至正常水平。CPL 对人乳腺癌（MCF-7）和肺癌（A-549）癌细胞系进行的 MTT 试验显示，在浓度为 128 和 256 µg/mL 时，分别具有 29.53%和 18.30%的抑制活性。
