Etoh T
Gan No Rinsho. 1985 Mar;31(3):299-302.
Monoclonal antibody (A 2 B 5) was raised against chick neural retina cells by Eisenbarth et al. We have studied, exclusively, A 2 B 5 for the immunological approach, and the following results were obtained. A 2 B 5 was shown to be IgG 2a subclass and reacted with all human neuroblastoma cell lines, NBGOTO, NB 1, TN-1 and C-NB1, in vitro by the 51Cr release cytotoxicity assay, but did not have cytotoxic activity for other tumor cell lines, C-1300 (A/J mouse neuroblastoma cell line), S1509a (A/J mouse induced sarcoma by methylcholanthrene) and P 39 (human malignant melanoma cell line). A 2 B 5 was titrated against constant numbers of human neuroblastoma cell lines. In the next experiment, detection of metastatic neuroblastoma cells in bone marrow, specimen and human neuroblastoma cell lines was undertaken by indirect immunofluorescence using A 2 B 5. All were visualized with binding A 2 B 5, and immunofluorescence could be seen on a clump of tumor cells binding A 2 B 5. Thus, A 2 B 5 may be particularly useful reagent for the diagnosis of bone metastasis of human neuroblastoma.
艾森巴思等人用鸡神经视网膜细胞制备了单克隆抗体（A 2 B 5）。我们专门研究了用于免疫方法的A 2 B 5，并得到了以下结果。A 2 B 5被证明是IgG 2a亚类，通过51Cr释放细胞毒性试验在体外与所有人类神经母细胞瘤细胞系NBGOTO、NB 1、TN - 1和C - NB1发生反应，但对其他肿瘤细胞系C - 1300（A/J小鼠神经母细胞瘤细胞系）、S1509a（A/J小鼠经甲基胆蒽诱导的肉瘤）和P 39（人类恶性黑色素瘤细胞系）没有细胞毒性活性。用恒定数量的人类神经母细胞瘤细胞系对A 2 B 5进行滴定。在接下来的实验中，使用A 2 B 5通过间接免疫荧光法对骨髓、标本和人类神经母细胞瘤细胞系中的转移性神经母细胞瘤细胞进行检测。所有细胞与A 2 B 5结合后都能被观察到，并且在一群与A 2 B 5结合的肿瘤细胞上可以看到免疫荧光。因此，A 2 B 5可能是诊断人类神经母细胞瘤骨转移的特别有用的试剂。
