Huang Shuhan, Rizzo Daniella, Wu Sherry Jingjing, Xu Qing, Ziane Leena, Alghamdi Norah, Stafford David A, Daigle Tanya L, Tasic Bosiljka, Zeng Hongkui, Ibrahim Leena Ali, Fishell Gord
Harvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA.
Program in Neuroscience, Harvard University, Cambridge, MA 02138, USA.
bioRxiv. 2024 Jun 11:2024.06.05.597539. doi: 10.1101/2024.06.05.597539.
Neurogliaform cells are a distinct type of GABAergic cortical interneurons known for their "volume transmission" output property. However, their activity and function within cortical circuits remain unclear. Here, we developed two genetic tools to target these neurons and examine their function in the primary visual cortex. We found that the spontaneous activity of neurogliaform cells positively correlated with locomotion. Silencing these neurons increased spontaneous activity during locomotion and impaired visual responses in L2/3 pyramidal neurons. Furthermore, the contrast-dependent visual response of neurogliaform cells varies with their laminar location and is constrained by their morphology and input connectivity. These findings demonstrate the importance of neurogliaform cells in regulating cortical behavioral state-dependent spontaneous activity and indicate that their functional engagement during visual stimuli is influenced by their laminar positioning and connectivity.
神经胶质样细胞是一种独特类型的γ-氨基丁酸能皮质中间神经元，以其“容积传递”输出特性而闻名。然而，它们在皮质回路中的活动和功能仍不清楚。在这里，我们开发了两种基因工具来靶向这些神经元，并研究它们在初级视觉皮层中的功能。我们发现神经胶质样细胞的自发活动与运动呈正相关。沉默这些神经元会增加运动过程中的自发活动，并损害L2/3锥体神经元的视觉反应。此外，神经胶质样细胞的对比度依赖性视觉反应随其层状位置而变化，并受其形态和输入连接性的限制。这些发现证明了神经胶质样细胞在调节皮质行为状态依赖性自发活动中的重要性，并表明它们在视觉刺激期间的功能参与受其层状定位和连接性的影响。
