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Effect of temperature and 4,6'-diamidine-2-phenylindole on restriction of supercoiled Col E1 DNA by Eco RI endonuclease.

作者信息

Stepień E, Filutowicz M, Fikus M

出版信息

Acta Biochim Pol. 1979;26(1-2):29-38.

PMID:388956
Abstract

Supercoiled Col E1 DNA is split by Eco RI endonuclease at 37 degrees C without intermediate formation of open circular DNA. Accumulation of this restriction product is observed at low temperature. The fluorescent dye, 4,6'-diamidine-2-phenylindole (DAPI) inhibits restriction by Eco RI endonuclease. This effect is due to the DAPI:DNA rather than to the DAPI:Eco RI interactions.

摘要

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引用本文的文献

1
Interaction of diamidino-2-phenylindole (DAPI) with natural and synthetic nucleic acids.二脒基-2-苯基吲哚（DAPI）与天然和合成核酸的相互作用。
Nucleic Acids Res. 1983 Dec 20;11(24):8861-76. doi: 10.1093/nar/11.24.8861.