二脒基-2-苯基吲哚（DAPI）与天然和合成核酸的相互作用。
Interaction of diamidino-2-phenylindole (DAPI) with natural and synthetic nucleic acids.
作者信息
Manzini G, Barcellona M L, Avitabile M, Quadrifoglio F
出版信息
Nucleic Acids Res. 1983 Dec 20;11(24):8861-76. doi: 10.1093/nar/11.24.8861.
Abstract
The interaction of DAPI with natural and synthetic polydeoxynucleotides of different base content and sequences was studied with circular dichroism, ultracentrifugation, viscosity and calorimetry. All the polymers show two types of binding. The strength of the interaction and its resistance to ionic strength are related to the content of AT clusters in the chain. On the other hand, sedimentation measurements rule out an intercalation mechanism. A model of DAPI interaction with DNA, similar to that displayed by distamycin and netropsin, is proposed.
摘要
利用圆二色性、超速离心、粘度和量热法研究了DAPI与不同碱基含量和序列的天然及合成聚脱氧核苷酸的相互作用。所有聚合物均表现出两种结合类型。相互作用的强度及其对离子强度的抗性与链中AT簇的含量有关。另一方面，沉降测量排除了嵌入机制。提出了一种DAPI与DNA相互作用的模型，类似于偏端霉素和纺锤菌素所表现出的模型。
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