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探索园艺作物体细胞克隆变异的意义。

Exploring the Significance of Somaclonal Variations in Horticultural Crops.

作者信息

Manchanda Pooja, Sharma Deepansh, Kaur Gurpreet, Kaur Harleen

机构信息

School of Agricultural Biotechnology, College of Agriculture, Punjab Agricultural University, Ludhiana, 141004, India.

Department of Plant Pathology, College of Agriculture, Punjab Agricultural University, Ludhiana, 141004, India.

出版信息

Mol Biotechnol. 2025 Jun;67(6):2185-2203. doi: 10.1007/s12033-024-01214-6. Epub 2024 Jun 19.

Abstract

Genetic and epigenetic variations produced via cell and tissue culture open up new sources of variability intra-species which can be used to improve crops. The use of in vitro generated somaclonal variations for selecting novel variants aids in the development of novel genotypes having desirable agronomic traits that can be released as varieties or utilized for breeding purposes. Horticultural crops give higher yield and productivity per unit area than other crops, as well as provide good economic returns which have led to an increase in their potential benefits throughout time. The last three to four decades have seen the selection and release of a number of valuable somaclonal variants, many of which possess remarkable features including disease resistance, high yield, improved nutritional quality and abiotic stress tolerance. Generating somaclonal variations has given breeders a novel alternative option for obtaining genetic diversity in horticultural crops and without advanced technologies. The variations introduced through tissue culture process, methods to determine and validate genetic changes in vitro regenerated plantlets, along with prospective application of such variations in horticultural crops' improvement are reviewed in the present work.

摘要

通过细胞和组织培养产生的遗传和表观遗传变异为种内变异开辟了新来源,可用于改良作物。利用体外产生的体细胞克隆变异来选择新变异,有助于开发具有理想农艺性状的新基因型,这些基因型可作为品种发布或用于育种目的。园艺作物单位面积产量和生产力高于其他作物,并且能带来良好的经济回报,这使得它们的潜在效益一直以来都在增加。在过去的三四十年里,人们筛选并发布了许多有价值的体细胞克隆变异体,其中许多具有显著特征,包括抗病性、高产、改善营养品质和非生物胁迫耐受性。产生体细胞克隆变异为育种者提供了一种在不借助先进技术的情况下获得园艺作物遗传多样性的新选择。本文综述了通过组织培养过程引入的变异、体外再生植株遗传变化的测定和验证方法,以及这些变异在园艺作物改良中的潜在应用。

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