Universidade Federal do Rio Grande do Norte, Programa de Pós-Graduação em Biologia Parasitária, Natal, RN, Brasil.
Universidade Federal de Minas Gerais, Departamento de Parasitologia, Belo Horizonte, MG, Brasil.
Mem Inst Oswaldo Cruz. 2024 Jun 17;119:e240013. doi: 10.1590/0074-02760240013. eCollection 2024.
The impact of Schistosoma mansoni infection over the immune response and the mechanisms involved in pathogenesis are not yet completely understood.
This study aimed to evaluate the expression of innate immune receptors in three distinct mouse lineages (BALB/c, C57BL/6 and Swiss) during experimental S. mansoni infection with LE strain.
The parasite burden, intestinal tissue oogram and presence of hepatic granulomas were evaluated at 7- and 12-weeks post infection (wpi). The mRNA expression for innate Toll-like receptors, Nod-like receptors, their adaptor molecules, and cytokines were determined at 2, 7 and 12 wpi in the hepatic tissue by real-time quantitative polymerase chain reaction (qPCR).
Swiss mice showed 100% of survival, had lower parasite burden and intestinal eggs, while infected BALB/c and C57BL/6 presented 80% and 90% of survival, respectively, higher parasite burden and intestinal eggs. The three mouse lineages displayed distinct patterns in the expression of innate immune receptors, their adaptor molecules and cytokines, at 2 and 7 wpi.
Our results suggest that the pathogenesis of S. mansoni infection is related to a dynamic early activation of innate immunity receptors and cytokines important for the control of developing worms.
曼氏血吸虫感染对免疫反应的影响及其发病机制中的相关作用尚未完全阐明。
本研究旨在评估 LE 株感染实验中，三种不同品系（BALB/c、C57BL/6 和瑞士）小鼠固有免疫受体的表达情况。
在感染后 7 周和 12 周时，评估寄生虫负荷、肠道组织学图谱和肝肉芽肿的存在情况。通过实时定量聚合酶链反应（qPCR），在肝组织中于 2、7 和 12 周时，测定固有 Toll 样受体、Nod 样受体、其衔接分子和细胞因子的 mRNA 表达情况。
瑞士小鼠的存活率为 100%，寄生虫负荷和肠道虫卵较少，而感染的 BALB/c 和 C57BL/6 的存活率分别为 80%和 90%，寄生虫负荷和肠道虫卵较多。在 2 周和 7 周时，三种小鼠品系在固有免疫受体、其衔接分子和细胞因子的表达模式上存在明显差异。
本研究结果表明，曼氏血吸虫感染的发病机制与固有免疫受体和细胞因子的早期动态激活有关，这些受体和细胞因子对控制发育中的蠕虫至关重要。
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