Neurological Disorders Research Center, Qatar Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation, PO Box 34110, Doha, Qatar.
Stem Cell Core, Qatar Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation, PO Box 34110, Doha, Qatar; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, 41522, Ismailia, Egypt.
Stem Cell Res. 2024 Sep;79:103470. doi: 10.1016/j.scr.2024.103470. Epub 2024 Jun 11.
Autism spectrum disorder (ASD) is a complex developmental disorder characterized by challenges with social interactions and restricted/repetitive behaviors. Here, we recruited nine Qatari children of Arab ethnicity (males, aged 2-4 years), including six ASD subjects (n = 3 mild-to-moderate ASD and n = 3 severe ASD) and three control subjects. We generated induced pluripotent stem cell (iPSC) lines from PBMC samples of these subjects using non-integrating Sendai viral vectors. These iPSC lines were fully characterized and exhibited pluripotency characteristics, normal karyotypes, and trilineage differentiation potential. These iPSC lines provide valuable cell models for understanding ASD pathophysiology and developing new therapeutics for ASD.
自闭症谱系障碍（ASD）是一种复杂的发育障碍，其特征是社交互动和受限/重复行为方面存在挑战。在这里，我们招募了 9 名具有阿拉伯血统的卡塔尔儿童（男性，年龄 2-4 岁），包括 6 名 ASD 受试者（n=3 名轻度至中度 ASD 和 n=3 名重度 ASD）和 3 名对照受试者。我们使用非整合性 Sendai 病毒载体从这些受试者的 PBMC 样本中生成诱导多能干细胞（iPSC）系。这些 iPSC 系经过充分表征，表现出多能性特征、正常核型和三系分化潜能。这些 iPSC 系为理解 ASD 病理生理学和开发 ASD 的新疗法提供了有价值的细胞模型。
