Patey G, Liston D, Rossier J
Neuropeptides. 1985 Feb;5(4-6):513-6. doi: 10.1016/0143-4179(85)90067-8.
Immunoblots combined with specific radioimmunoassays (RIAs) have been used to visualize simultaneously all the enkephalin-containing peptides (ECPs) present in a crude extract of bovine adrenal medulla. They have allowed the characterization of a new high molecular weight ECP which has a molecular weight of 23.3 kDalton, contains the amino-terminal part of proenkephalin and ends with the sequence of Leu-enkephalin at its carboxy-terminus.
免疫印迹法结合特定的放射免疫测定法（RIAs）已被用于同时可视化牛肾上腺髓质粗提物中存在的所有含脑啡肽的肽（ECP）。这些方法已使一种新的高分子量ECP得以鉴定，其分子量为23.3千道尔顿，包含前脑啡肽的氨基末端部分，并且在其羧基末端以亮氨酸脑啡肽序列结尾。
