Patey G, Liston D, Rossier J
FEBS Lett. 1984 Jul 9;172(2):303-8. doi: 10.1016/0014-5793(84)81146-1.
Immunoblotting combined with radioimmunoassays (RIAs) directed specifically towards certain sequences of the proenkephalin molecule has been used to characterize the enkephalin-containing peptides (ECPs) present in the bovine adrenal medulla. Immunoblotting allowed the simultaneous visualization of all ECPs present in a crude extract of this gland. Combining this technique with RIAs we have been able to characterize a new high molecular mass ECP, a 23.3-kDa protein which contains the amino-terminal part of proenkephalin and ends with the sequence of Leu-enkephalin at its carboxy-terminus.
免疫印迹法与专门针对脑啡肽原分子特定序列的放射免疫测定法（RIA）相结合，已被用于鉴定牛肾上腺髓质中存在的含脑啡肽肽（ECP）。免疫印迹法能同时可视化该腺体粗提物中存在的所有ECP。将这项技术与RIA相结合，我们得以鉴定出一种新的高分子量ECP，即一种23.3 kDa的蛋白质，它包含脑啡肽原的氨基末端部分，在其羧基末端以亮氨酸脑啡肽序列结尾。
