Fu Junqiang, Wu Tingting, Kuang Xuan, Xu Kun, Ren Xiang, Wu Dan, Ma Hongmin, Li Faying, Liu Lei, Wei Qin
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; School of Chemistry and Pharmaceutical Engineering Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271016, PR China.
Talanta. 2024 Sep 1;277:126346. doi: 10.1016/j.talanta.2024.126346. Epub 2024 Jun 12.
A novel dual-mode biosensor was constructed for the ultrasensitive detection of neuron-specific enolase (NSE), utilizing Tb-Cu MOF@Au nanozyme as the signal label to effectively quench the photoelectrochemical (PEC) signals of BiO/BiS/AgBiS composites and initiate fluorescent (FL) signals. First, BiO/BiS/AgBiS heterojunction with excellent photoelectric activity was selected as the substrate material to provide a stable photocurrent. The well-matched energy levels significantly enhanced the separation and transfer of photogenerated carriers. Second, a strategy of consuming ascorbic acid (AA) by Tb-Cu MOF@Au nanozyme was introduced to improve the sensitivity of the PEC/FL biosensor. Tb-Cu MOF@Au not only could catalyze the oxidation of AA, but the steric effect further reduced the contact of AA with the substrate. More importantly, in the presence of HO, a significant fluorescence was produced from Tb sensitized by the oxidation products of AA. Based on the above strategies, a highly stable and sensitive dual-mode biosensor was proposed for accurate NSE determination. Third, the developed dual-mode biosensor demonstrated excellent performance in detecting NSE. In this study, the PEC method demonstrated a wide detection range from 0.00005 to 200 ng/mL with a low detection limit of 20 fg/mL. The FL method exhibited a linear range from 0.001 to 200 ng/mL with a detection limit of 0.65 pg/mL. The designed biosensor showed potential practical implications in the accurate detection of disease markers.
构建了一种新型双模式生物传感器，用于超灵敏检测神经元特异性烯醇化酶（NSE），利用Tb-Cu MOF@Au纳米酶作为信号标签，有效淬灭BiO/BiS/AgBiS复合材料的光电化学（PEC）信号并引发荧光（FL）信号。首先，选择具有优异光电活性的BiO/BiS/AgBiS异质结作为基底材料，以提供稳定的光电流。良好匹配的能级显著增强了光生载流子的分离和转移。其次，引入了一种利用Tb-Cu MOF@Au纳米酶消耗抗坏血酸（AA）的策略，以提高PEC/FL生物传感器的灵敏度。Tb-Cu MOF@Au不仅可以催化AA的氧化，而且空间位阻效应进一步减少了AA与基底的接触。更重要的是，在HO存在的情况下，AA的氧化产物敏化Tb产生了显著的荧光。基于上述策略，提出了一种用于准确测定NSE的高稳定性和高灵敏度双模式生物传感器。第三，所开发的双模式生物传感器在检测NSE方面表现出优异的性能。在本研究中，PEC方法的检测范围为0.00005至200 ng/mL，检测限低至20 fg/mL。FL方法的线性范围为0.001至200 ng/mL，检测限为0.65 pg/mL。所设计的生物传感器在疾病标志物的准确检测中显示出潜在的实际应用价值。
