School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Talanta. 2024 Dec 1;280:126705. doi: 10.1016/j.talanta.2024.126705. Epub 2024 Aug 13.
In this paper, BiS/AgBiS composite nanomaterials and PDA@Ag@N-CQDs were synthesized, and used as substrates and second antibody label respectively to construct a sandwich photoelectrochemical (PEC) sensor. The upconversion luminescence effect of N-CQDs can convert long wavelength light into short wavelength light that can be utilized by the substrate material, which can provide additional excitation light energy for the substrate material and further enhance the photoelectric response. Besides, Ag has SPR effect and can also promote electron transfer. The proposed sandwich immunosensor achieves detection of NSE in the concentration range of 0.001 ng mL to 100 ng mL, with a detection limit of 0.28 pg mL (S/N = 3). What's more, the proposed sensor also exhibits good stability, selectivity, as well as reproducibility, indicating its promising application prospects.
本文合成了 BiS/AgBiS 复合纳米材料和 PDA@Ag@N-CQDs，并分别用作基底和第二抗体标记物，构建了三明治光电化学（PEC）传感器。N-CQDs 的上转换发光效应可以将长波长光转换为基底材料可利用的短波长光，可为基底材料提供额外的激发光能，进一步增强光电响应。此外，Ag 具有 SPR 效应，也可以促进电子转移。所提出的三明治免疫传感器实现了对 NSE 在 0.001ng/mL 至 100ng/mL 浓度范围内的检测，检测限为 0.28pg/mL（S/N=3）。此外，该传感器还表现出良好的稳定性、选择性和重现性，表明其具有广阔的应用前景。
