College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China.
Int J Biol Macromol. 2024 Jul;273(Pt 2):133225. doi: 10.1016/j.ijbiomac.2024.133225. Epub 2024 Jun 17.
This work aimed to investigate the antibacterial ability and potential mechanism of chitosan grafted gentisate acid derivatives (CS-g-GA) against Pseudomonas fluorescens. The results showed that CS-g-GA had a significant suppressive impact on the growth of Pseudomonas fluorescens, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 0.64 mg/mL and 1.28 mg/mL, respectively. Results of scanning electron microscopy (SEM) and alkaline phosphatase (AKPase) confirmed that CS-g-GA destroyed the cell structure thereby causing the leakage of intracellular components. In addition, 1 × MIC of CS-g-GA could significantly inhibit the formation of biofilms, and 74.78 % mature biofilm and 86.21 % extracellular polysaccharide of Pseudomonas fluorescens were eradicated by CS-g-GA at 2 × MIC. The results on the respiratory energy metabolism system and antioxidant system demonstrated that CS-g-GA caused respiratory disturbance and energy limitation by influencing the key enzyme activities. It could also bind to DNA and affect genetic metabolism. From this, it could be seen that CS-g-GA had the potential to control foodborne contamination of Pseudomonas fluorescens by attacking multiple targets.
本研究旨在探究接枝没食子酸衍生物壳聚糖（CS-g-GA）对荧光假单胞菌的抗菌能力及其潜在机制。结果表明，CS-g-GA 对荧光假单胞菌的生长具有显著的抑制作用，其最小抑菌浓度（MIC）和最小杀菌浓度（MBC）分别为 0.64mg/mL 和 1.28mg/mL。扫描电子显微镜（SEM）和碱性磷酸酶（AKPase）的结果证实，CS-g-GA 破坏了细胞结构，导致细胞内成分泄漏。此外，1×MIC 的 CS-g-GA 可显著抑制生物膜的形成，2×MIC 的 CS-g-GA 可消除 74.78%的成熟生物膜和 86.21%的荧光假单胞菌胞外多糖。对呼吸能量代谢系统和抗氧化系统的研究表明，CS-g-GA 通过影响关键酶活性引起呼吸障碍和能量限制，还可以与 DNA 结合，影响遗传代谢。由此可见，CS-g-GA 可通过攻击多个靶位，有望控制食源污染荧光假单胞菌。
