绿原酸接枝壳聚糖对金黄色葡萄球菌的抗菌和抗生物膜活性。
Antimicrobial and anti-biofilm activities of chlorogenic acid grafted chitosan against Staphylococcus aureus.
机构信息
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai, China; National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, China.
出版信息
Microb Pathog. 2022 Dec;173(Pt A):105748. doi: 10.1016/j.micpath.2022.105748. Epub 2022 Sep 5.
In this work, Chitosan-grafted-chlorogenic acid (CS-g-CA) was prepared by the carbodiimide method. The purpose of this study was to investigate the antibacterial and anti-biofilm activity of CS-g-CA against Staphylococcus aureus (S. aureus). The minimum inhibitory concentration (MIC) of CS-g-CA against S. aureus was identified as 0.625 mg/mL. S. aureus treated with 1/2 × MIC of CS-g-CA had a longer logarithmic growth phase than that of the CK group, while 1 × MIC and 2 × MIC inhibited the growth of bacteria. The damaging effect of CS-g-CA on bacterial cells was analyzed by measuring the activity of cellular antioxidant enzymes (Catalase (CAT) and Glutathione peroxidase (GSH-Px)) and intracellular enzymes (alkaline phosphatase (AKPase) and adenosine triphosphatase (ATPase)). The results of DNA gel electrophoresis illustrated that CS-g-CA disrupted the normal metabolism of bacteria. Scanning electron microscopy (SEM) results showed that S. aureus shrank and died under CS-g-CA treatment. 1 × MIC of CS-g-CA can significantly inhibit the formation of biofilms, and 1/2 × MIC of CS-g-CA control the swimming speed of S. aureus. In addition, 77.53% mature biofilm and 60.62% extracellular polysaccharide (EPS) in the mature biofilm of S. aureus were eradicated by CS-g-CA at 2 × MIC. Confocal laser scanning microscopy (CLSM) observation further confirmed these results. Therefore, CS-g-CA was an antimicrobial and antibiofilm agent to control S. aureus, which can effectively controlling the growth of S. aureus in food, thereby preventing the occurrence of food-borne diseases.
在这项工作中，通过碳二亚胺法制备了壳聚糖接枝绿原酸（CS-g-CA）。本研究旨在研究 CS-g-CA 对金黄色葡萄球菌（S. aureus）的抗菌和抗生物膜活性。CS-g-CA 对 S. aureus 的最小抑菌浓度（MIC）确定为 0.625mg/mL。用 1/2×MIC 的 CS-g-CA 处理的 S. aureus 的对数生长期比 CK 组长，而 1×MIC 和 2×MIC 抑制了细菌的生长。通过测量细胞抗氧化酶（过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px））和细胞内酶（碱性磷酸酶（AKPase）和三磷酸腺苷酶（ATPase））的活性来分析 CS-g-CA 对细菌细胞的破坏作用。DNA 凝胶电泳结果表明，CS-g-CA 破坏了细菌的正常代谢。扫描电子显微镜（SEM）结果表明，S. aureus 在 CS-g-CA 处理下收缩和死亡。1×MIC 的 CS-g-CA 能显著抑制生物膜的形成，而 1/2×MIC 的 CS-g-CA 能控制 S. aureus 的游动速度。此外，2×MIC 的 CS-g-CA 可消除 S. aureus 成熟生物膜中 77.53%的成熟生物膜和 60.62%的胞外多糖（EPS）。共聚焦激光扫描显微镜（CLSM）观察进一步证实了这些结果。因此，CS-g-CA 是一种抗微生物和抗生物膜剂，可有效控制食品中 S. aureus 的生长，从而预防食源性疾病的发生。
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