School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China.
School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China; Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen 518055, China; State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; Joint Laboratory of Urban High Strength Wastewater Treatment and Resource Utilization, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.
Bioresour Technol. 2024 Aug;406:130947. doi: 10.1016/j.biortech.2024.130947. Epub 2024 Jun 17.
Intermittent hydroxylamine (NHOH) dosing strategy was applied to enhance the stability of partial nitrification and total nitrogen (N) removal efficiency (TNRE) in a continuous-flow process. The results showed 2 mg/L of NHOH dosing (once every 6 h) could maintain stably partial nitrification with nitrite accumulation rate (NAR) of 91.6 % and TNRE of 92.6 %. The typical cycle suggested NHOH dosing could promote simultaneous nitrification-denitrification (SND) and endogenous denitrification (END) while inhibit exogenous denitrification (EXD). Nitrification characteristics indicated the NHOH dosing enhanced stability of partial nitrification by suppressing specific nitrite oxidation rate (SNOR), Nitrospira and nitrite oxidoreductase enzyme (Nxr). The microbial community suggested the aerobic denitrfiers, denitrifying glycogen accumulating organisms (DGAOs) and traditional denitrfiers were the potential contributor for advanced N removal. Moreover, NHOH dosage was positively associated with NAR, SND and END. Overall, this study offers a feasible strategy to maintain sustainably partial nitrification that has great application potential.
间歇羟胺（NHOH）投加策略被应用于提高连续流工艺中部分硝化和总氮（TN）去除效率（TNRE）的稳定性。结果表明，2mg/L 的 NHOH 投加（每 6 小时投加一次）可以稳定地维持部分硝化，亚硝酸盐积累率（NAR）为 91.6%，TNRE 为 92.6%。典型的周期表明，NHOH 投加可以促进同时硝化-反硝化（SND）和内源性反硝化（END），同时抑制外源性反硝化（EXD）。硝化特性表明，NHOH 投加通过抑制特定的亚硝酸盐氧化速率（SNOR）、硝化螺旋菌和亚硝酸盐氧化还原酶（Nxr）来增强部分硝化的稳定性。微生物群落表明，好氧反硝化菌、反硝化聚磷菌（DGAOs）和传统反硝化菌是实现高级氮去除的潜在贡献者。此外，NHOH 投加量与 NAR、SND 和 END 呈正相关。总的来说，本研究提供了一种维持可持续部分硝化的可行策略，具有很大的应用潜力。
