Laifi-Necibi Nermine, Amor Nabil, Merella Paolo, Mohammed Osama Badri, Medini Lamia
Faculté des Sciences de Tunis, Laboratoire Diversité, Gestion et Conservation des Systèmes Biologiques, Université de Tunis El Manar, Tunis, Tunisia.
Higher Institute of Applied Biological Sciences of Tunis, University Tunis EL Manar, Tunis, Tunisia.
Mitochondrial DNA A DNA Mapp Seq Anal. 2025 Jan-Mar;35(1-2):1-11. doi: 10.1080/24701394.2024.2363350. Epub 2024 Jun 20.
Barcoding studies have provided significant insights into phylogenetic relationships among species belonging to the genus (Crustacea, Isopoda). Herein the diversity of the Italian sea slater from Tunisia is studied for the first time. Samples were collected from 18 localities in Tunisia, and the analysis included previously published sequences from Italy and Greece available in GenBank. Bayesian and Maximum Likelihood phylogenetic analyses were carried out using a fragment of the mitochondrial COI gene. Putative cryptic species were explored using the 'barcode gap' approach in the software ASAP. A genetic landscape shape analysis was carried out using the program Alleles in Space. The analyses revealed highly divergent and well-supported clades of dispersed across Tunisia (Clades A1 and A2), Greece (Clade B) and Italy (Clades C1 and C2). High genetic dissimilarity among clades suggested that constitute a cryptic species complex. Divergence among different lineages (Clades A, B and C) occurred around 7-4.5 Ma. The detected high genetic distances among clades did not result from atypical mitochondrial DNAs or intracellular infection by bacteria. The complex history of the Mediterranean Sea appears to have played a significant role in shaping the phylogeographic pattern of . Additional morphological and molecular studies are needed to confirm the existence of cryptic species in in Mediterranean.
条形码研究为属于[具体属名未给出]属（甲壳纲，等足目）的物种间系统发育关系提供了重要见解。本文首次对来自突尼斯的意大利海虱的多样性进行了研究。样本从突尼斯的18个地点采集，分析还包括GenBank中先前发表的来自意大利和希腊的序列。使用线粒体COI基因片段进行了贝叶斯和最大似然系统发育分析。在软件ASAP中使用“条形码间隙”方法探索了假定的隐存种。使用程序Alleles in Space进行了遗传景观形状分析。分析揭示了分散在突尼斯（A1和A2分支）、希腊（B分支）和意大利（C1和C2分支）的[具体物种名未给出]的高度分化且得到有力支持的分支。分支间的高遗传差异表明[具体物种名未给出]构成了一个隐存种复合体。不同[具体物种名未给出]谱系（A、B和C分支）之间的分化发生在大约7 - 450万年前。检测到的分支间的高遗传距离并非由非典型线粒体DNA或[具体细菌名未给出]细菌的细胞内感染导致。地中海复杂的历史似乎在塑造[具体物种名未给出]的系统地理格局中起到了重要作用。需要进一步的形态学和分子研究来确认地中海[具体物种名未给出]中隐存种的存在。
