Clevenger W, Reeves R, Pall M L
Plasmid. 1985 May;13(3):193-9. doi: 10.1016/0147-619x(85)90042-3.
Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the process of unequal crossing-over have been hampered by the lack of genetic systems allowing specific selection for cells that have undergone such unequal crossing-over. We report here on the construction of plasmids designed to provide specific selection of unequal crossing-over. One such plasmid was studied in Escherichia coli. We show that kanamycin resistance is generated, as predicted, by the expected unequal crossover event.
不等交换参与了果蝇、细菌和动物病毒等多种遗传系统中的基因复制和缺失。有人提出，在培养的动物细胞的基因扩增过程以及致癌过程中，不等交换以不等姐妹染色单体交换的形式参与其中。由于缺乏能够对经历了这种不等交换的细胞进行特异性选择的遗传系统，对不等交换过程的研究受到了阻碍。我们在此报告旨在提供不等交换特异性选择的质粒的构建。在大肠杆菌中研究了其中一种这样的质粒。我们表明，正如预期的那样，通过预期的不等交换事件产生了卡那霉素抗性。
