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[体细胞中同源DNA重组的特征。I. 含有氨基糖苷磷酸核糖基转移酶基因缺失变体的DNA质粒的构建]

[The characteristics of homologous DNA recombination in somatic cells. I. The construction of a DNA plasmid containing deletion variants of the aminoglycoside phosphoribosyltransferase gene].

作者信息

Romanov S R, Abramian D S, Kaminskaia E V, Glebov O K

出版信息

Tsitologiia. 1993;35(4):93-106.

PMID:8328029
Abstract

A plasmid DNA family containing deletion variants of Neo-gene has been constructed for investigation of somatic cell homologous recombination. Experimental data are presented provided by studies of extrachromosomal recombination, recombination following plasmid transfection into somatic cells, and recombination in the nuclear extracts. The opportunities of application of the plasmid constructions in the different pattern homologous recombination experiments performed on mammalian cells have been discussed.

摘要

为研究体细胞同源重组构建了一个包含新霉素基因缺失变体的质粒DNA家族。本文展示了通过对染色体外重组、质粒转染体细胞后的重组以及核提取物中的重组研究获得的实验数据。还讨论了这些质粒构建体在哺乳动物细胞上进行的不同模式同源重组实验中的应用机会。

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