Kriek E
Cancer Lett. 1979 Jul;7(2-3):141-6. doi: 10.1016/s0304-3835(79)80109-3.
The substitution reaction products of N-acetoxy-2-acetylaminofluorene (N-AcO-AAF) and the N-sulfate (potassium salt) of N-hydroxy-4-acetyl-aminobiphenyl (N-OSO3K-AABP) with DNA from calf thymus were determined after reaction in buffered solutions of 0.10 M NaCl at pH values from 4--9. In the case of N-AcO-AAF, the ratio of N-(guanin-8-yl)-2-acetylaminofluorene (N-(guanine-8-yl)-AAF) to 3-(guanin-N2-yl)-2-acetylaminofluorene (3-(guanin-N2-yl)-AAF) increased 2.2 times over the entire pH range studied, starting at pH 9. With the N-OSO3K-AABP, the total substitution of guanine was much lower (22--34 times) as compared with N-AcO-AAF, and the ratio of N-(guanin-8-yl)-4-acetylaminobiphenyl to 3-(guanin-N2-yl)-4-acetylaminobiphenyl was not affected by a change in pH of the reaction medium. As expected, heat-denatured DNA reacted more extensively with both esters, but an increase in substitution was much more pronounced for the biphenyl derivative (9 times) than for the fluorene compound (2.8 times). Degradation, denaturation or interstrand cross-linking of DNA were not observed under the reaction conditions employed.
在pH值为4 - 9的0.10 M NaCl缓冲溶液中反应后，测定了N - 乙酰氧基 - 2 - 乙酰氨基芴（N - AcO - AAF）和N - 羟基 - 4 - 乙酰氨基联苯的N - 硫酸盐（钾盐）（N - OSO₃K - AABP）与小牛胸腺DNA的取代反应产物。对于N - AcO - AAF，从pH 9开始，在所研究的整个pH范围内，N - （鸟嘌呤 - 8 - 基） - 2 - 乙酰氨基芴（N - （鸟嘌呤 - 8 - 基） - AAF）与3 - （鸟嘌呤 - N₂ - 基） - 2 - 乙酰氨基芴（3 - （鸟嘌呤 - N₂ - 基） - AAF）的比例增加了2.2倍。对于N - OSO₃K - AABP，与N - AcO - AAF相比，鸟嘌呤的总取代量要低得多（22 - 34倍），并且反应介质pH的变化不影响N - （鸟嘌呤 - 8 - 基） - 4 - 乙酰氨基联苯与3 - （鸟嘌呤 - N₂ - 基） - 4 - 乙酰氨基联苯的比例。正如预期的那样，热变性DNA与两种酯的反应更广泛，但联苯衍生物的取代增加（9倍）比芴化合物（2.8倍）更明显。在所采用的反应条件下未观察到DNA的降解、变性或链间交联。
