B型和Z型DNA对N-乙酰氧基-2-乙酰氨基芴的反应活性。
Reactivity of B and Z-DNA towards N-acetoxy-2-acetylaminofluorene.
作者信息
Spodheim-Maurizot M, Malfoy B, Saint-Ruf G
出版信息
Nucleic Acids Res. 1982 Jul 24;10(14):4423-30. doi: 10.1093/nar/10.14.4423.
Abstract
Poly d(G-C) d(G-C) in B-form, on one hand, and poly d(G-br5C). poly d(G-br5C) and poly d(G-m5C) . poly d(G-m5C) in Z-form, on another hand, were treated with N-AcO-[3H]AAF and the kinetics of these reactions were followed by radioactivity. Covalent binding of carcinogen to the polymers was evaluated after separation of the reacted polymers from non-reacted carcinogen by thin-layer chromatography. We found that B-form polymer reacts twice faster than the Z-form polymers. Proportions of main adducts in the three polymers are almost the same. Results are discussed in relation to the calculated electrostatic potential minima and steric accessibility at the reactive site (1, 2).
摘要
一方面，处于B型的聚d(G-C)·d(G-C)，另一方面，处于Z型的聚d(G-br5C)·聚d(G-br5C)和聚d(G-m5C)·聚d(G-m5C)，用N-乙酰氧基-[³H]AAF处理，并用放射性追踪这些反应的动力学。通过薄层色谱法将反应后的聚合物与未反应的致癌物分离后，评估致癌物与聚合物的共价结合。我们发现B型聚合物的反应速度比Z型聚合物快两倍。三种聚合物中主要加合物的比例几乎相同。结合计算出的反应位点处的静电势最小值和空间可及性对结果进行了讨论(1,2)。
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