Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Am J Emerg Med. 2024 Aug;82:107-116. doi: 10.1016/j.ajem.2024.06.009. Epub 2024 Jun 13.
Tempol, a synthetic antioxidant compound, has received significant attention for its potential therapeutic applications in recent years, especially against ischemia/reperfusion (I/R) injury. The aim of the present research was to assess the protective effects of Tempol on testicular I/R injury caused by testicular torsion and detorsion (T/D) in rats.
The subjects were divided into five groups: sham, testicular T/D, testicular T/D with Tempol treatment at 50 and 100 mg/kg, and healthy rats treated with Tempol at 100 mg/kg. Testicular torsion was induced by rotating the left testicles for 2 h, followed by detorsion for 24 h. Testicular tissues were evaluated for gene expression, oxidative stress markers, and histopathology, epididymal sperms were stained and analyzed, and blood serum samples were collected to measure the testosterone hormone.
The results showed that testicular I/R caused a significant decrease in sperm velocity parameters, viability, and count, as well as an increase in abnormal sperms (p < 0.05). However, treatment with Tempol significantly improved these parameters (p < 0.05). Histopathological analysis revealed severe damage to the testicular tissues, but treatment with Tempol improved the structural integrity of the seminiferous tubules. Testicular I/R also resulted in increased oxidative stress index and decreased testosterone levels significantly (p < 0.05), but Tempol administration mitigated these effects significantly (p < 0.05). Furthermore, the expression of Bax and Bcl, genes associated with apoptosis, were significantly altered by testicular I/R (p < 0.05), but Tempol prevented these changes significantly (p < 0.05).
These findings provide strong evidence that Tempol can effectively prevent testicular I/R injury.
近年来，作为一种合成抗氧化剂化合物，Tempol 因其在缺血/再灌注（I/R）损伤方面的潜在治疗应用而受到广泛关注。本研究旨在评估 Tempol 对大鼠睾丸扭转/复位（T/D）引起的睾丸 I/R 损伤的保护作用。
将研究对象分为五组：假手术组、睾丸 T/D 组、睾丸 T/D 后 Tempol 治疗 50 和 100mg/kg 组、健康大鼠 Tempol 治疗 100mg/kg 组。通过旋转左侧睾丸 2 小时诱导睾丸扭转，然后复位 24 小时。评估睾丸组织的基因表达、氧化应激标志物和组织病理学，对附睾精子进行染色和分析，并采集血清样本以测量睾丸激素水平。
结果显示，睾丸 I/R 导致精子速度参数、活力和计数显著下降，异常精子增加（p<0.05）。然而，Tempol 治疗显著改善了这些参数（p<0.05）。组织病理学分析显示睾丸组织严重损伤，但 Tempol 治疗改善了生精小管的结构完整性。睾丸 I/R 还导致氧化应激指数显著增加和睾丸激素水平显著降低（p<0.05），但 Tempol 给药显著减轻了这些影响（p<0.05）。此外，与细胞凋亡相关的 Bax 和 Bcl 基因的表达也因睾丸 I/R 而显著改变（p<0.05），但 Tempol 显著阻止了这些变化（p<0.05）。
这些发现提供了强有力的证据表明 Tempol 可以有效预防睾丸 I/R 损伤。
