Leszczynski D, Renkonen R, Häyry P
Scand J Immunol. 1985 Apr;21(4):355-60. doi: 10.1111/j.1365-3083.1985.tb01441.x.
The turnover rate of rat renal dendritic cells was analysed by irradiation and bone marrow transplantation and by visualizing the dendritic cells in frozen sections of renal tissue via double indirect immunofluorescence and peroxidase assays. Interstitial dendritic cells disappear from rat renal tissue shortly after irradiation; after 7 days they can be seen again. However, since the renal proximal tubular cells also simultaneously lose and regain their class II contents, we assume that irradiation has resulted in the disappearance of the Ia antigen, not the dendritic cells proper. After allogeneic bone marrow transplantation, bone marrow-derived dendritic cells appear in renal tissue between days 7 and 10, indicating a relatively fast turnover rate of these cells in vivo.
通过照射和骨髓移植，并通过双重间接免疫荧光和过氧化物酶测定法对肾组织冰冻切片中的树突状细胞进行可视化，分析了大鼠肾树突状细胞的周转率。照射后不久，间质树突状细胞从大鼠肾组织中消失；7天后又可见到它们。然而，由于肾近端小管细胞同时也失去并重新获得其II类成分，我们认为照射导致Ia抗原消失，而非树突状细胞本身消失。同种异体骨髓移植后，骨髓来源的树突状细胞在第7至10天之间出现在肾组织中，表明这些细胞在体内的周转率相对较快。
