Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Liquor Making Biology Technology and Application of Key Laboratory of Sichuan Province, College of Bioengineering, Sichuan University of Science and Engineering, 188 University Town, Yi bin, Yibin, 644000, PR China.
World J Microbiol Biotechnol. 2024 Jun 21;40(8):246. doi: 10.1007/s11274-024-04056-1.
Saccharomyces cerevisiae, the primary microorganism involved in ethanol production, is hindered by the accumulation of ethanol, leading to reduced ethanol production. In this study, we employed histidine-modified FeO nanoparticles (His-FeO) for the first time, to the best of our knowledge, as a method to enhance ethanol yield during the S. cerevisiae fermentation process. The results demonstrated that exposing S. cerevisiae cells to FeO nanoparticles (FeO NPs) led to increased cell proliferation and glucose consumption. Moreover, the introduction of His-FeO significantly boosted ethanol content by 17.3% (p < 0.05) during fermentation. Subsequent findings indicated that the increase in ethanol content was associated with enhanced ethanol tolerance and improved electron transport efficiency. This study provided evidence for the positive effects of His-FeO on S. cerevisiae cells and proposed a straightforward approach to enhance ethanol production in S. cerevisiae fermentation. The mediation of improved ethanol tolerance offers significant potential in the fermentation and bioenergy sectors.
酿酒酵母是参与乙醇生产的主要微生物，但由于乙醇的积累会对其产生抑制作用，从而导致乙醇产量降低。在本研究中，据我们所知，首次使用组氨酸修饰的 FeO 纳米粒子（His-FeO）作为一种方法来提高酿酒酵母发酵过程中的乙醇产量。结果表明，使酿酒酵母细胞暴露于 FeO 纳米粒子（FeO NPs）中会导致细胞增殖和葡萄糖消耗增加。此外，在发酵过程中，引入 His-FeO 可使乙醇含量显著提高 17.3%（p<0.05）。随后的研究结果表明，乙醇含量的增加与乙醇耐受性的提高和电子传递效率的改善有关。本研究为 His-FeO 对酿酒酵母细胞的积极影响提供了证据，并提出了一种简单的方法来提高酿酒酵母发酵中的乙醇产量。改善乙醇耐受性的介导在发酵和生物能源领域具有重要的应用潜力。
