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拟南芥 AGB1 通过 bZIP17 介导的未折叠蛋白反应参与盐胁迫响应。

Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response.

机构信息

Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115201, Taiwan.

出版信息

BMC Plant Biol. 2024 Jun 21;24(1):586. doi: 10.1186/s12870-024-05296-x.

DOI:10.1186/s12870-024-05296-x
PMID:38902609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11191249/
Abstract

BACKGROUND

Plant heterotrimeric G proteins respond to various environmental stresses, including high salinity. It is known that Gβ subunit AGB1 functions in maintaining local and systemic Na/K homeostasis to accommodate ionic toxicity under salt stress. However, whether AGB1 contributes to regulating gene expression for seedling's survival under high salinity remains unclear.

RESULTS

We showed that AGB1-Venus localized to nuclei when facing excessive salt, and the induction of a set of bZIP17-dependent salt stress-responsive genes was reduced in the agb1 mutant. We confirmed both genetic and physical interactions of AGB1 and bZIP17 in plant salinity response by comparing salt responses in the single and double mutants of agb1 and bzip17 and by BiFC assay, respectively. In addition, we show that AGB1 depletion decreases nuclei-localization of transgenic mRFP-bZIP17 under salt stress, as shown in s1p s2p double mutant in the Agrobacteria-mediated transient mRFP-bZIP17 expression in young seedlings.

CONCLUSIONS

Our results indicate that AGB1 functions in S1P and/or S2P-mediated proteolytic processing of bZIP17 under salt stress to regulate the induction of salinity-responsive gene expression.

摘要

背景

植物异源三聚体 G 蛋白对各种环境胁迫（包括高盐度）做出响应。已知 Gβ亚基 AGB1 在维持局部和全身 Na/K 离子平衡以适应盐胁迫下的离子毒性方面发挥作用。然而，AGB1 是否有助于调节高盐度下幼苗生存的基因表达仍不清楚。

结果

我们发现，面对过量盐分时，AGB1-Venus 定位于细胞核，并且在 agb1 突变体中，一组 bZIP17 依赖性盐胁迫响应基因的诱导减少。我们通过比较 agb1 和 bzip17 的单突变体和双突变体的盐响应，以及 BiFC 测定，分别证实了 AGB1 和 bZIP17 在植物盐响应中的遗传和物理相互作用。此外，我们还表明，AGB1 的缺失减少了转基因 mRFP-bZIP17 在盐胁迫下的核定位，正如在 Agrobacterium 介导的年轻幼苗中转基因 mRFP-bZIP17 的瞬时表达的 s1p s2p 双突变体中所显示的那样。

结论

我们的结果表明，AGB1 在盐胁迫下 S1P 和/或 S2P 介导的 bZIP17 蛋白水解加工中发挥作用，以调节盐胁迫响应基因表达的诱导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/7cf33ca904ee/12870_2024_5296_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/9071d8c11d4b/12870_2024_5296_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/e8f11fb97242/12870_2024_5296_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/2343a6c91e72/12870_2024_5296_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/56e254e3ba1b/12870_2024_5296_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/ce02b53edf62/12870_2024_5296_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/7cf33ca904ee/12870_2024_5296_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/9071d8c11d4b/12870_2024_5296_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/e8f11fb97242/12870_2024_5296_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/2343a6c91e72/12870_2024_5296_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/56e254e3ba1b/12870_2024_5296_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/ce02b53edf62/12870_2024_5296_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2209/11191249/7cf33ca904ee/12870_2024_5296_Fig6_HTML.jpg

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