Abdulsahib Waleed K, Al-Radeef Mohanad Y
Department of Pharmacology and Toxicology, College of Pharmacy, Al Farahidi University, Baghdad, Iraq.
Department of Clinical Pharmacy, College of Pharmacy, Tikrit University, Tikrit, Iraq.
J Adv Pharm Technol Res. 2024 Apr-Jun;15(2):63-69. doi: 10.4103/JAPTR.JAPTR_496_23. Epub 2024 May 6.
Globally, an estimated 50 million people are affected by epilepsy, a persistent, noncommunicable neurological ailment. Quercetin (QR) is a prevalent flavonoid substance extensively dispersed throughout agricultural life. In a pilocarpine (PILO)-induced epilepsy model in mice, this investigation aimed to determine whether QR has an antiepileptic effect and explore its putative mechanism of action. Fifty mice were allocated into seven groups, with six in every group. The first group received physiological saline, the second group was given diazepam (1 mg/kg), and four groups were administered QR at 50, 100, 150, and 200 mg/kg, respectively. The seventh group (the induction group) received normal saline. After 30 min, all groups were injected intraperitoneally with PILO. The impact of QR on motor coordination was assessed using the rotarod test, while measures such as latency to first seizure, generalized tonic-clonic seizures (GTCS), number of convulsions, and mortality were recorded. Serum samples were collected through the retro-orbital route to measure prostaglandin E2 (PGE2) and interleukin 1 beta (IL-1β) levels. QR showed no significant difference in motor impairment, but increased duration until the initial seizure occurred and declined the mortality rate, duration of GTCS, and incidence of convulsions. All doses of QR significantly reduced PGE2 levels ( ≤ 0.05). However, QR's effect on IL-1β reduction was statistically insignificant ( > 0.05). QR's capacity to inhibit PILO-induced epilepsy by decreasing IL-1 and PGE2 levels is supported by this study. The results of this work indicate that QR could have a function to treat acute epilepsy.
全球范围内，估计有5000万人受癫痫影响，这是一种持续性的非传染性神经疾病。槲皮素（QR）是一种广泛存在的类黄酮物质，在整个农业生产中广泛分布。在小鼠匹罗卡品（PILO）诱导的癫痫模型中，本研究旨在确定QR是否具有抗癫痫作用，并探讨其可能的作用机制。将50只小鼠分为七组，每组六只。第一组接受生理盐水，第二组给予地西泮（1mg/kg），四组分别给予50、100、150和200mg/kg的QR。第七组（诱导组）接受生理盐水。30分钟后，所有组腹腔注射PILO。使用转棒试验评估QR对运动协调性的影响，同时记录首次癫痫发作潜伏期、全身强直阵挛性发作（GTCS）、惊厥次数和死亡率等指标。通过眶后途径采集血清样本，以测量前列腺素E2（PGE2）和白细胞介素1β（IL-1β）水平。QR在运动功能损害方面无显著差异，但延长了首次癫痫发作的时间，并降低了死亡率、GTCS持续时间和惊厥发生率。所有剂量的QR均显著降低了PGE2水平（≤0.05）。然而，QR对IL-1β降低的作用在统计学上不显著（>0.05）。本研究支持QR通过降低IL-1和PGE2水平来抑制PILO诱导的癫痫的能力。这项工作的结果表明，QR可能具有治疗急性癫痫的作用。
