Pinatel M C
Ann Biol Clin (Paris). 1985;43(1):49-53.
Sampling precautions are particularly important in the study of sperm: the length of sexual abstinence, complete collection of the ejaculate, sampling by masturbation in the laboratory. The examination of sperm consists of different steps: measurement of the ejaculate volume, evaluation of the mobility, speed, and propulsion direction of the spermatozoa, study of spermatozoon concentration, estimation of the percentage of live spermatozoa, morphological examination of the spermatozoa, including an enumeration of the abnormal forms, evaluation of spermatozoon survival in vitro for 24 h by monitoring the percentage of mobility every 2 h. This examination can be complemented by biochemical and enzymatic assays of: fructose, zinc, carnitine, citric acid, acid phosphatase, alpha glucosidase, which serve as tracers of associated secretions.
在精子研究中，采样预防措施尤为重要：禁欲时间、精液的完整采集、在实验室通过手淫采样。精子检查包括不同步骤：测量精液体积、评估精子的活力、速度和推进方向、研究精子浓度、估算活精子百分比、精子形态学检查，包括异常形态的计数、通过每2小时监测活力百分比评估精子在体外24小时的存活率。这项检查可以通过以下生化和酶学检测来补充：果糖、锌、肉碱、柠檬酸、酸性磷酸酶、α-葡萄糖苷酶，这些可作为相关分泌物的追踪指标。
