State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
University of Chinese Academy of Sciences, Beijing, China.
Sci Data. 2024 Jun 21;11(1):658. doi: 10.1038/s41597-024-03503-w.
Euphorbia, one of the largest genera of flowering plants, is well-known for containing many biofuel crops. Euphorbia tirucalli, an evergreen succulent mainly native to the Africa continent but cultivated worldwide, is a promising petroleum plant with high tolerance to drought and salt stress. However, the exploration of such an important plant resource is severely hampered by the lack of a reference genome. Here, we present the chromosome-level genome assembly of E. tirucalli using PacBio HiFi sequencing and Hi-C technology. Its genome size was approximately 745.62 Mb, with a contig N50 of 74.16 Mb. A total of 743.63 Mb (99.73%) of the assembled sequences were anchored to 10 chromosomes with a complete BUSCO score of 97.80%. Genome annotation revealed 26,304 protein-coding genes, and 76.37% of the genome was identified as repeat elements. The high-quality genome provides valuable genetic resources that would be useful for unraveling the genetic mechanisms of biofuel synthesis and evolutionary adaptation of E. tirucalli.
大戟属是开花植物中最大的属之一，以包含许多生物燃料作物而闻名。绿玉树，一种原产于非洲大陆但在全球范围内种植的常绿肉质植物，是一种很有前途的石油植物，具有很强的耐旱和耐盐能力。然而，由于缺乏参考基因组，对这种重要植物资源的探索受到了严重阻碍。在这里，我们使用 PacBio HiFi 测序和 Hi-C 技术展示了绿玉树的染色体水平基因组组装。其基因组大小约为 745.62Mb，contig N50 为 74.16Mb。总共 743.63Mb（99.73%）的组装序列锚定在 10 条染色体上，完整的 BUSCO 评分为 97.80%。基因组注释揭示了 26304 个蛋白质编码基因，76.37%的基因组被鉴定为重复元件。高质量的基因组提供了有价值的遗传资源，这将有助于揭示绿玉树生物燃料合成的遗传机制和进化适应。
