[55株大肠杆菌对兔肠上皮细胞的黏附、定居因子CFA/I和CFA/II的存在及产毒素能力的比较研究]

[Comparative study of adherence to rabbit enterocytes, presence of colonization factors CFA/I and CFA/II and toxinogenesis of 55 strains of Escherichia coli].

作者信息

Germani Y, Brethes B, Bégaud E, Moreau J P

出版信息

Ann Inst Pasteur Microbiol (1985). 1985 Mar-Apr;136A(2):203-12. doi: 10.1016/s0769-2609(85)80059-4.

Abstract

Fifty-five strains of Escherichia coli isolated from 51 faeces of Melanesian children with acute diarrhoea in New Caledonia were studied; three diarrhoeas were bloody. For each strain, haemagglutination type, adhesion to rabbit enterocytes, serotype, production of heat-labile (LT) or heat-stable (ST) toxins and identification of colonization factor antigens CFA/I or CFA/II were determined. We identified 48 strains able to attach to rabbit enterocytes; 27 produced enterotoxins (21 strains LT+ and 6 ST+) and 19 had CFA (13 CFA/I and 6 CFA/II). Five serotypes were identified: O6, O78, O80, O114 and O127:B8. One strain, O127:B8, which was able to attach to enterocytes, had CFA/I and produced LT toxin.

摘要

对从新喀里多尼亚患急性腹泻的美拉尼西亚儿童的51份粪便中分离出的55株大肠杆菌进行了研究；其中3例腹泻为血性腹泻。对每株菌株测定了血凝类型、对兔肠细胞的黏附性、血清型、不耐热（LT）或耐热（ST）毒素的产生以及定居因子抗原CFA/I或CFA/II的鉴定。我们鉴定出48株能够黏附于兔肠细胞的菌株；27株产生肠毒素（21株LT+和6株ST+），19株具有CFA（13株CFA/I和6株CFA/II）。鉴定出了5种血清型：O6、O78、O80、O114和O127:B8。一株O127:B8菌株能够黏附于肠细胞，并具有CFA/I且产生LT毒素。