Agüero M E, Reyes L, Prado V, Orskov I, Orskov F, Cabello F C
J Clin Microbiol. 1985 Oct;22(4):576-81. doi: 10.1128/jcm.22.4.576-581.1985.
The incidence of enterotoxigenic Escherichia coli (ETEC) was investigated in 95 E. coli strains isolated from 48 infants with diarrhea in Santiago, Chile. By using standard biological assays and DNA-DNA hybridization procedures, ETEC was found in 31.2% of the cases: 14 strains produced heat-stable enterotoxin (ST) only, three strains produced heat-labile enterotoxin (LT) and ST, and two strains produced LT only. DNA probes detected all enterotoxin producers except one ST-producing strain. The ST strains hybridized with one or both of the human ST probes (ST Ib and ST A2). Two of the LT-ST strains hybridized with the ST Ia and ST Ib probes, and the third strain did not hybridize with any of the ST probes. Only the ST group expressed multiple resistance (85.7%) and colonization factor antigen I (CFA I) (92.8%); CFA II was found in two of three LT-ST strains. The O153:H45 serotype was found in 10 of 14 ST strains, and O6:K15:H16 was found in one LT strain and in two LT-ST strains. These findings suggest that ETEC, especially strains that produce ST, may be an important cause of diarrhea among Chilean infants.
对从智利圣地亚哥48名腹泻婴儿中分离出的95株大肠杆菌进行了产肠毒素大肠杆菌（ETEC）的发病率调查。通过标准生物学检测和DNA-DNA杂交程序，在31.2%的病例中发现了ETEC：14株仅产生耐热肠毒素（ST），3株产生不耐热肠毒素（LT）和ST，2株仅产生LT。DNA探针检测到了除1株产ST菌株外的所有产肠毒素菌株。ST菌株与一种或两种人ST探针（ST Ib和ST A2）杂交。其中两株LT-ST菌株与ST Ia和ST Ib探针杂交，第三株菌株未与任何ST探针杂交。只有ST组表现出多重耐药性（85.7%）和定植因子抗原I（CFA I）（92.8%）；在三株LT-ST菌株中的两株中发现了CFA II。14株ST菌株中有10株为O153:H45血清型，1株LT菌株和2株LT-ST菌株为O6:K15:H16血清型。这些发现表明，ETEC，尤其是产ST的菌株，可能是智利婴儿腹泻的一个重要原因。
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