Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA.
Methods Mol Biol. 2024;2822:227-243. doi: 10.1007/978-1-0716-3918-4_16.
Single-cell RNA sequencing (scRNA-seq) enables the measurement of RNA expressed from individual cells within a tissue or population. RNA expression profiles may be used to draw conclusions about cellular states, cell subtypes within the population, responses to perturbations, and cellular behavior in the context of disease. Here we describe a method for scRNA-seq via single-cell encapsulation and capture of the polyadenosine tails at the 3' end of mRNA transcripts combined with cell and molecular barcoding, allowing for the sequencing of 3' untranslated regions in order to identify expressed genes from a cell.
单细胞 RNA 测序（scRNA-seq）可用于测量组织或群体中单个细胞内表达的 RNA。RNA 表达谱可用于根据细胞状态、群体中的细胞亚型、对干扰的反应以及疾病背景下的细胞行为得出结论。本文中，我们描述了一种通过单细胞包封和捕获 mRNA 转录物 3' 端聚腺苷酸尾巴，并结合细胞和分子条形码的方法来进行 scRNA-seq，这允许对 3' 非翻译区进行测序，以鉴定来自单个细胞的表达基因。
