Seliuchenko O A, Chertov O Iu, Lipkin V M
Bioorg Khim. 1985 Apr;11(4):480-91.
Chemical modification of DNA-dependent RNA polymerase with monoiod-[14C]acetic acid and N-[14C]ethylmaleimide has been carried out and position of superficial and functionally important as well as enzymatically non-significant, exposed cysteine residues have been localised in the enzyme. Topography of cysteine residues in the RNA-polymerase alpha-subunit is thoroughly studied. The results are summarized in a model.
已使用单碘 - [¹⁴C]乙酸和N - [¹⁴C]乙基马来酰亚胺对依赖DNA的RNA聚合酶进行了化学修饰,并确定了该酶中表面的、功能重要的以及酶学上无显著意义的暴露半胱氨酸残基的位置。对RNA聚合酶α亚基中半胱氨酸残基的拓扑结构进行了深入研究。研究结果总结在一个模型中。