Department of Cell and Molecular Biology, BMC, Uppsala University, SE-751 24, Uppsala, Sweden.
Sci Data. 2024 Jun 22;11(1):678. doi: 10.1038/s41597-024-03513-8.
Dicytostelium firmibasis is a member of Dictyostelia, a group of social amoebae that upon starvation display aggregative multicellularity where the amoebae transition from uni- to multicellular life. The D. firmibasis genome assembly that is currently available is of limited use due to its low contiguity, large number of undetermined bases, and lack of annotations. Here we used Nanopore long read sequencing, complemented with Illumina sequencing, and developmental transcriptomics as well as small RNA-sequencing, to present a new, fully annotated, chromosome-level D. firmibasis genome assembly. The new assembly contains no undetermined bases, and consists mainly of six large contigs representing the chromosomes, as well as a complete mitochondrial genome. This new genome assembly will be a valuable tool, allowing comprehensive comparison to Dictyostelium discoideum, the dictyostelid genetically tractable model. Further, the new genome will be important for studies of evolutionary processes governing the transition from unicellular to multicellular organisms and will aid in the sequencing and annotation of other dictyostelids genomes, many of which are currently of poor quality.
坚实基齿阿米巴（Dicytostelium firmibasis）是粘菌门的一种，当处于饥饿状态时，粘菌门中的群居变形虫会表现出聚集的多细胞性，即从单细胞生命过渡到多细胞生命。目前可用的 D. firmibasis 基因组组装由于其连续性低、大量未确定碱基和缺乏注释而用途有限。在这里，我们使用纳米孔长读测序，辅以 Illumina 测序以及发育转录组学和小 RNA 测序，提供了一个新的、完全注释的、染色体水平的 D. firmibasis 基因组组装。新的组装没有未确定的碱基，主要由六个代表染色体的大连续体以及一个完整的线粒体基因组组成。这个新的基因组组装将是一个有价值的工具，允许与粘菌门遗传上可操作的模式生物 D. discoideum 进行全面比较。此外，新的基因组对于研究从单细胞生物向多细胞生物过渡的进化过程将非常重要，并有助于测序和注释其他粘菌门基因组，其中许多目前质量较差。
